Assessment of the Diversity of Pseudomonas spp. and Fusarium spp. in Radix pseudostellariae Rhizosphere under Monoculture by Combining DGGE and Quantitative PCR.
Assessment of the Diversity of Pseudomonas spp. and Fusarium spp. in Radix pseudostellariae Rhizosphere under Monoculture by Combining DGGE and Quantitative PCR.
复制标题
假单胞菌属多样性的评估。
DOI：
10.3389/fmicb.2017.01748
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发表时间：
2017
影响因子：
5.2
通讯作者：
Lin W
中科院分区：
文献类型：
--
作者：
Chen J;Wu L;Xiao Z;Wu Y;Wu H;Qin X;Wang J;Wei X;Khan MU;Lin S;Lin W
太子参是一种多年生滋补药用植物，具有很高的药用价值。但连续单作造成产量和品质严重下降。通过3年的田间试验，研究了长时间单养对红葡萄生长的抑制作用。太子参利用DGGE技术研究了土壤镰刀菌和假单胞菌群落沿着3年梯度的结构和多样性变化。结果表明，扩大单作能显著提高镰刀菌的多样性，而假单胞菌属（Pseudomonas spp.）多样性定量PCR分析表明，尖孢镰刀菌的显着增加，但假单胞菌的下降。此外，拮抗假单胞菌的丰度。对F.在连续单作土壤中，尖孢菌的数量显著减少。与土壤中检测到的相同比例的酚酸混合物可以促进病原真菌菌丝和孢子的生长。而对拮抗假单胞菌CJ 313的生长有抑制作用。此外，植物生测结果表明，假单胞菌CJ 313具有良好的保护R.太子参感染F.尖孢菌属因此，本研究表明，扩大单一栽培的R。太子参能改变植物根际镰刀菌和假单胞菌的群落结构，导致根际拮抗微生物水平相对较低，而病原微生物水平相对较高。
Radix pseudostellariae is a perennial tonic medicinal plant, with high medicinal value. However, consecutive monoculture of this plant in the same field results in serious decrease in both yield and quality. In this study, a 3-year field experiment was performed to identify the inhibitory effect of growth caused by prolonged monoculture of R. pseudostellariae. DGGE analysis was used to explore the shifts in the structure and diversity of soil Fusarium and Pseudomonas communities along a 3-year gradient of monoculture. The results demonstrated that extended monoculture significantly boosted the diversity of Fusarium spp., but declined Pseudomonas spp. diversity. Quantitative PCR analysis showed a significant increase in Fusarium oxysporum, but a decline in Pseudomonas spp. Furthermore, abundance of antagonistic Pseudomonas spp. possessing antagonistic ability toward F. oxysporum significantly decreased in consecutively monocultured soils. Phenolic acid mixture at the same ratio as detected in soil could boost mycelial and sporular growth of pathogenic F. oxysporum while inhibit the growth of antagonistic Pseudomonas sp. CJ313. Moreover, plant bioassays showed that Pseudomonas sp. CJ313 had a good performance that protected R. pseudostellariae from infection by F. oxysporum. In conclusion, this study demonstrated that extended monoculture of R. pseudostellariae could alter the Fusarium and Pseudomonas communities in the plant rhizosphere, leading to relatively low level of antagonistic microorganisms, but with relatively high level of pathogenic microorganisms.
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影响因子：
56.9
作者：
Lebeis, Sarah L.;Paredes, Sur Herrera;Dangl, Jeffery L.
通讯作者：
Dangl, Jeffery L.
影响因子：
4.2
作者：
Clausen, GB;Larsen, L;Aamand, J
通讯作者：
Aamand, J
影响因子：
2.6
作者：
Gorlach-Lira, K.;Stefaniak, O.
通讯作者：
Stefaniak, O.
影响因子：
3.2
作者：
Samson, R;Shafik, H;Gardan, L
通讯作者：
Gardan, L
影响因子：
5.1
作者：
ROGERS, SO;BENDICH, AJ
通讯作者：
BENDICH, AJ