Molecular comparison and evolutionary analyses of VP1 nucleotide sequences of new African human enterovirus 71 isolates reveal a wide genetic diversity.
Molecular comparison and evolutionary analyses of VP1 nucleotide sequences of new African human enterovirus 71 isolates reveal a wide genetic diversity.
复制标题
新非洲人肠病毒71个分离株的VP1核苷酸序列的分子比较和进化分析揭示了广泛的遗传多样性。
DOI：
10.1371/journal.pone.0090624
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bailly JL
中科院分区：
文献类型：
--
作者：
Bessaud M;Razafindratsimandresy R;Nougairède A;Joffret ML;Deshpande JM;Dubot-Pérès A;Héraud JM;de Lamballerie X;Delpeyroux F;Bailly JL
根据编码VP 1衣壳蛋白的1D基因之间的遗传差异，大多数人肠道病毒71（EV-A71）的流行株主要分为三个基因组（A至C）。本研究的目的是进一步了解EV-A71基因组的多样性，特别是那些来自非洲国家，包括马达加斯加。我们通过大量比较从GenBank中收集的3，346个VP 1核苷酸序列对最近的EV-A71分离株进行了分类。遗传距离分析和系统发育分析表明，部分分离株不属于A-C基因组，而聚为另外3个基因组，包括1个印度基因组（基因组D）和2个非洲基因组（基因组E和F）。我们的贝叶斯系统发育分析提供了一致的数据，表明基因组D分离株与基因组E的成员共享最近的共同祖先，而基因组F的分离株从与基因组B的成员共享最近的共同祖先进化而来。我们的研究结果揭示了EV-A71分离株之间存在的广泛多样性，并表明循环基因组的数量可能被低估，特别是在EV-A71流行病学研究不足的发展中国家。
Most circulating strains of Human enterovirus 71 (EV-A71) have been classified primarily into three genogroups (A to C) on the basis of genetic divergence between the 1D gene, which encodes the VP1 capsid protein. The aim of the present study was to provide further insights into the diversity of the EV-A71 genogroups following the recent description of highly divergent isolates, in particular those from African countries, including Madagascar. We classified recent EV-A71 isolates by a large comparison of 3,346 VP1 nucleotidic sequences collected from GenBank. Analysis of genetic distances and phylogenetic investigations indicated that some recently-reported isolates did not fall into the genogroups A-C and clustered into three additional genogroups, including one Indian genogroup (genogroup D) and 2 African ones (E and F). Our Bayesian phylogenetic analysis provided consistent data showing that the genogroup D isolates share a recent common ancestor with the members of genogroup E, while the isolates of genogroup F evolved from a recent common ancestor shared with the members of the genogroup B. Our results reveal the wide diversity that exists among EV-A71 isolates and suggest that the number of circulating genogroups is probably underestimated, particularly in developing countries where EV-A71 epidemiology has been poorly studied.
登录
查看更多内容
影响因子：
3.8
作者：
Lukashev, AN;Lashkevich, VA;Ilonen, J
通讯作者：
Ilonen, J
影响因子：
5.4
作者：
Bouslama, Lamjed;Nasri, Dorsaf;Pillet, Sylvie
通讯作者：
Pillet, Sylvie
影响因子：
5.4
作者：
Oberste, MS;Maher, K;Pallansch, MA
通讯作者：
Pallansch, MA
影响因子：
5.4
作者：
Brown, BA;Oberste, MS;Pallansch, MA
通讯作者：
Pallansch, MA
影响因子：
9.8
作者：
Drummond AJ;Ho SY;Phillips MJ;Rambaut A
通讯作者：
Rambaut A