Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage.
Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage.
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DOI：
10.3390/biomedicines5030057
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发表时间：
2017-09-18
期刊：
影响因子：
4.7
通讯作者：
Kawase T
中科院分区：
文献类型：
--
作者：
Kawabata H;Isobe K;Watanabe T;Okudera T;Nakamura M;Suzuki M;Ryu J;Kitamura Y;Okudera H;Okuda K;Nakata K;Kawase T
富含血小板的纤维蛋白样基质（PRFM）通常在现场制备并立即用于再生治疗。尽管如此，为了满足保存 PRFM 而不降低质量的临床需要，我们开发了一种从短期储存的全血 (WB) 样本中制备 PRFM 的方法。在本研究中，为了评估实际的储存期限，我们将 WB 样本的储存时间从 2 天延长至 7 天，并评估了所得 PRFM 的质量。用酸性-柠檬酸盐-葡萄糖收集的WB样品在环境温度下轻轻搅拌保存。为了制备 PRFM，将储存的 WB 样品与 CaCl2 在玻璃管中混合并离心。分别通过扫描电子显微镜 (SEM)、流式细胞术和酶联免疫吸附测定 (ELISA) 检查纤维蛋白纤维网络、CD41 和 CD62P 表达以及血小板衍生生长因子-BB (PDGF-BB) 水平。长期储存对血细胞计数或测试的血小板功能没有显着影响。由此产生的 PRFM 在视觉上与新鲜制备的 PRFM 相同。 PDGF-BB水平并未以时间依赖性方式显着降低。然而，纤维蛋白纤维在储存后逐渐变细。尽管凝血活性可能会减弱，但我们建议可以通过我们之前开发的方法将 WB 样品储存长达 7 天来制备 PRFM，而不会明显降低质量。
The platelet-rich fibrin–like matrix (PRFM) is usually prepared onsite and immediately used for regenerative therapy. Nonetheless, to meet the clinical necessity of preserving the PRFM without quality deterioration, we developed a method for preparation of PRFMs from short-term-stored whole blood (WB) samples. In this study, to evaluate the practical expiration date of storage, we extended the storage time of WB samples from 2 to 7 days and assessed the quality of the resulting PRFMs. WB samples collected with acid-citrate-dextrose were stored with gentle agitation at ambient temperature. To prepare PRFMs, the stored WB samples were mixed with CaCl2 in glass tubes and centrifuged. Fibrin fiber networks, CD41 and CD62P expression, and Platelet Derived Growth Factor-BB (PDGF-BB) levels were examined by scanning electron microscopy (SEM), flow cytometry, and an Enzyme-Linked ImmunoSorbent Assay (ELISA), respectively. Long-term storage had no significant effect on either blood cell counts or platelet functions tested. The resulting PRFMs were visually identical to freshly prepared ones. PDGF-BB levels did not markedly decrease in a time-dependent manner. However, fibrin fibers gradually became thinner after storage. Although the coagulation activity may diminish, we propose that PRFMs can be prepared—without evident loss of quality—from WB samples stored for up to 7 days by our previously developed method.
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影响因子：
1.8
作者：
Nishiyama K;Okudera T;Watanabe T;Isobe K;Suzuki M;Masuki H;Okudera H;Uematsu K;Nakata K;Kawase T
通讯作者：
Kawase T
影响因子：
2.7
作者：
Isobe K;Watanebe T;Kawabata H;Kitamura Y;Okudera T;Okudera H;Uematsu K;Okuda K;Nakata K;Tanaka T;Kawase T
通讯作者：
Kawase T
影响因子：
2.7
作者：
Isobe K;Suzuki M;Watanabe T;Kitamura Y;Suzuki T;Kawabata H;Nakamura M;Okudera T;Okudera H;Uematsu K;Nakata K;Tanaka T;Kawase T
通讯作者：
Kawase T
影响因子：
1.9
作者：
Moore, Gary W.;Maloney, James C.;Najafi, Mitra F.
通讯作者：
Najafi, Mitra F.
影响因子：
2.5
作者：
Rodella, Luigi Fabrizio;Favero, Gaia;Rezzani, Rita
通讯作者：
Rezzani, Rita