The Effect of Pedal Peptide-Type Neuropeptide on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus.
The Effect of Pedal Peptide-Type Neuropeptide on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus.
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足肽型神经肽对仿刺参运动行为和肌肉生理的影响
DOI：
10.3389/fphys.2020.559348
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发表时间：
2020
影响因子：
4
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Ding K;Zhang L;Fan X;Guo X;Liu X;Yang H
神经肽是存在于神经组织中的内源性活性物质，参与动物系统的行为和生理过程。动物的运动行为是捕食、逃逸、繁殖等行为的基础，而神经肽在运动行为中起着重要作用。本研究探讨了足肽型神经肽（PDP）在仿刺参（Apostichopus）运动行为过程中的作用。对A.用红外摄像机记录PDP给药前后的肌张力变化，并采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）研究PDP给药后肌肉的生理变化。结果表明，PDP在注射后7 h显著延长了A的累计运动时间，使A的平均运动速度和最大运动速度分别降低了16.90%和14.22%; - 是的肌肉代谢组学研究结果表明，某些代谢产物的显著变化与海参的运动行为有关。磷脂酰乙醇胺（PE）和磷脂酰胆碱（PC）的减少可能导致溶血磷脂酰胆碱（lysoPC）和溶血磷脂酰乙醇胺（lysoPE）的增加，提示肌细胞膜流动性和通透性的改变，从而影响肌细胞的生理和功能，最终改变运动行为。此外，花生四烯酸（ARA）水平的升高可能通过激活K+通道影响肌细胞的信号转导，或通过提高肌细胞对Ca ~（2+）的敏感性而导致海参纵行肌的收缩。ARA还参与亚油酸代谢，这是PDP给药后唯一显著干扰的途径。因此，PDP参与了海参运动行为的调节，降低PE和PC，增加lysoPC、lysoPE和ARA可能是PDP对海参行为影响的潜在生理机制。- 是的
Neuropeptides are endogenous active substances that are present in nervous tissues and participate in behavioral and physiological processes of the animal system. Locomotor behavior is basic to predation, escape, reproduction in animals, and neuropeptides play an important role in locomotion. In this study, the function of pedal peptide-type neuropeptide (PDP) in the process of locomotor behavior of the sea cucumber Apostichopus japonicus was evaluated. The locomotor behavior of A. japonicus was recorded by infrared camera before and after PDP administration, and muscle physiology was studied by ultra performance liquid chromatography and quadrupole time-off-light mass spectrometry (UPLC-Q-TOF-MS) to clarify the potential physiological mechanisms. The results showed that PDP enhanced the cumulative duration of moving significantly at the 7th h after injection, and reduced the mean and maximum velocity by 16.90 and 14.22% in A. japonicus. The data of muscle metabolomics suggested that some significantly changed metabolites were related to locomotor behavior of sea cucumbers. The decreases of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) might result in the increases of lysophosphatidylcholines (lysoPC) and lysophosphatidylethanolamine (lysoPE), and suggested the change of fluidity and permeability in the muscle cell membrane, which would affect the physiology and function of muscle cells, and finally alter the locomotor behavior. In addition, the increased level of arachidonic acid (ARA) might activate K+ ion channels and then affect the signaling of muscle cells, or promote the sensitivity of muscle cells to Ca2+ and then result in the contractility of longitudinal muscles in sea cucumbers. ARA was also involved in the linoleic acid metabolism which was the only pathway that disturbed significantly after PDP administration. In conclusion, PDP participated in the regulation of locomotor behavior in the sea cucumber, and the decreased PE and PC, increased lysoPC, lysoPE and ARA might be the potential physiological mechanisms that responsible for behavioral effects of PDP in A. japonicus.
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DOI：
10.1016/j.tem.2016.05.007
发表时间：
2016-08
期刊：
Trends in endocrinology and metabolism: TEM
影响因子：
--
作者：
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通讯作者：
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影响因子：
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发表时间：
1999-04-01
期刊：
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY AND OCCUPATIONAL PHYSIOLOGY
影响因子：
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作者：
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通讯作者：
Glatz, JFC
DOI：
10.1002/cne.24371
发表时间：
2018-04-01
期刊：
The Journal of comparative neurology
影响因子：
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作者：
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通讯作者：
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鉴定出充当肌肉松弛剂的新型海星神经肽。
影响因子：
4.7
作者：
Kim CH;Kim EJ;Go HJ;Oh HY;Lin M;Elphick MR;Park NG
通讯作者：
Park NG