In Vivo Characterization of a Red Light-Activated Vasodilation: A Photobiomodulation Study.
In Vivo Characterization of a Red Light-Activated Vasodilation: A Photobiomodulation Study.
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DOI：
10.3389/fphys.2022.880158
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发表时间：
2022
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
一氧化氮依赖性血管舒张是恢复缺血组织血流的有效机制。先前，我们建立了离体小鼠模型，其中红光（670 nm）通过内皮衍生的血管活性物质促进血管舒张，所述血管活性物质含有可还原为一氧化氮的官能团。在本研究中，我们研究了这种血管扩张剂在体内通过测量血液流动与激光多普勒灌注成像小鼠。血管舒张的一氧化氮前体分析血浆和肌肉与三碘依赖性化学发光。首先，在670 nm（50 mW/cm 2）下对后肢中的3 cm 2区域进行5-10 min照射产生最佳血管舒张。照射的四头肌组织中的一氧化氮前体通过光处理的强度和持续时间从123 ± 18 pmol/g组织显著降低到平均90 ± 17 pmol/g组织，而在未暴露的对照后肢中保持稳定（137 ± 21 pmol/g组织）。第二，在光暴露终止后30分钟，血流量仍然升高。一氧化氮前体的含量显着增加了50%的辐射，然后耗尽在血浆中，而保持稳定的后肢肌肉。第三，为了模拟人类外周动脉疾病，在小鼠的近端股动脉上插入一个淀粉缩窄器，导致流量显着减少。重复光治疗14天，实现了稳定和显着增加的灌注在收缩的肢体。我们的研究结果强烈支持670 nm光可以通过释放血管活性一氧化氮前体物质来调节导管血管的扩张，并可能在未来为腿部血流受损的个体提供简单的家庭治疗。
Nitric oxide dependent vasodilation is an effective mechanism for restoring blood flow to ischemic tissues. Previously, we established an ex vivo murine model whereby red light (670 nm) facilitates vasodilation via an endothelium derived vasoactive species which contains a functional group that can be reduced to nitric oxide. In the present study we investigated this vasodilator in vivo by measuring blood flow with Laser Doppler Perfusion imaging in mice. The vasodilatory nitric oxide precursor was analyzed in plasma and muscle with triiodide-dependent chemiluminescence. First, a 5–10 min irradiation of a 3 cm2 area in the hind limb at 670 nm (50 mW/cm2) produced optimal vasodilation. The nitric oxide precursor in the irradiated quadriceps tissue decreased significantly from 123 ± 18 pmol/g tissue by both intensity and duration of light treatment to an average of 90 ± 17 pmol/g tissue, while stayed steady (137 ± 21 pmol/g tissue) in unexposed control hindlimb. Second, the blood flow remained elevated 30 min after termination of the light exposure. The nitric oxide precursor content significantly increased by 50% by irradiation then depleted in plasma, while remained stable in the hindlimb muscle. Third, to mimic human peripheral artery disease, an ameroid constrictor was inserted on the proximal femoral artery of mice and caused a significant reduction of flow. Repeated light treatment for 14 days achieved steady and significant increase of perfusion in the constricted limb. Our results strongly support 670 nm light can regulate dilation of conduit vessel by releasing a vasoactive nitric oxide precursor species and may offer a simple home-based therapy in the future to individuals with impaired blood flow in the leg.
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DOI：
10.1016/j.bbacli.2016.09.002
发表时间：
2016-12
期刊：
BBA clinical
影响因子：
--
作者：
Hamblin, Michael R
通讯作者：
Hamblin, Michael R
影响因子：
11.4
作者：
Keszler, Agnes;Lindemer, Brian;Lohr, Nicole L.
通讯作者：
Lohr, Nicole L.
影响因子：
2.1
作者：
Buzinari, Tereza C.;de Moraes, Thiago F.;Rodrigues, Gerson J.
通讯作者：
Rodrigues, Gerson J.
影响因子：
3.8
作者：
FURCHGOTT, R F;EHRREICH, S J;GREENBLATT, E
通讯作者：
GREENBLATT, E
影响因子：
5
作者：
Lohr, Nicole L.;Keszler, Agnes;Pratt, Phillip;Bienengraber, Martin;Warltier, David C.;Hogg, Neil
通讯作者：
Hogg, Neil