Impact of Scaffold Micro and Macro Architecture on Schwann Cell Proliferation under Dynamic Conditions in a Rotating Wall Vessel Bioreactor.
Impact of Scaffold Micro and Macro Architecture on Schwann Cell Proliferation under Dynamic Conditions in a Rotating Wall Vessel Bioreactor.
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DOI：
10.1016/j.msec.2010.04.001
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发表时间：
2011-01-01
影响因子：
7.9
通讯作者：
Yu, Xiaojun
中科院分区：
文献类型：
--
作者：
Valmikinathan, Chandra M.;Hoffman, John;Yu, Xiaojun
关键词：
在过去的十年中，组织工程已经成为一种强大的替代方法来再生由于创伤或肿瘤而失去的组织。有证据表明，与植入后依赖细胞化的支架相比，含有雪旺细胞的支架在体内具有更好的性能。然而，由于来自患者自身的细胞供应有限，已经采取了几种方法来提高细胞增殖率，以产生完整和均匀的支架细胞化。最常见的方法是应用生物反应器来提高细胞增殖率，从而减少在植入前获得足够数量的胶质细胞所需的时间。在这项研究中，我们展示了旋转壁生物反应器系统的应用，用于研究雪旺细胞在溶剂铸造和盐浸技术制备的纳米纤维螺旋形支架上的增殖。该支架材料为聚己内酯（PCL），具有理想的力学性能，降解后不会产生酸性副产物。螺旋支架表面涂有由静电纺丝生产的排列或随机纳米纤维，以提供模仿天然细胞外基质和基本接触引导线索的基质。在第4天的时间点，与静态培养条件相比，在旋转壁生物反应器中观察到开放结构纳米纤维螺旋支架的细胞增殖率增强。然而，与静态条件相比，在同一时间点，其他当代支架结构（如管状和圆柱形支架）上的细胞增殖率显示生物反应器中的细胞增殖率降低。此外，旋转壁生物反应器不会改变雪旺细胞在排列的含纳米纤维支架上的取向或表型，其中，细胞沿着支架的长度保持排列。因此，这些开放结构的螺旋支架与雪旺细胞预培养，在生物反应器中，可能会缩短移植周围神经再生所需的时间。
Over the last decade tissue engineering has emerged as a powerful alternative to regenerate lost tissues owing to trauma or tumor. Evidence shows that Schwann cell containing scaffolds have improved performance in vivo as compared to scaffolds that depend on cellularization post implantation. However, owing to limited supply of cells from the patients themselves, several approaches have been taken to enhance cell proliferation rates to produce complete and uniform cellularization of scaffolds. The most common approach is the application of a bioreactor to enhance cell proliferation rate and therefore reduce the time needed to obtain sufficiently significant number of glial cells, prior to implantation. In this study, we show the application of a rotating wall bioreactor system for studying Schwann cell proliferation on nanofibrous spiral shaped scaffolds, prepared by solvent casting and salt leaching techniques. The scaffolds were fabricated from polycaprolactone (PCL), which has ideal mechanical properties and upon degradation does not produce acidic byproducts. The spiral scaffolds were coated with aligned or random nanofibers, produced by electrospinning, to provide a substrate that mimics the native extracellular matrix and the essential contact guidance cues. At the 4 day time point, an enhanced rate of cell proliferation was observed on the open structured nanofibrous spiral scaffolds in a rotating wall bioreactor, as compared to static culture conditions. However, the cell proliferation rate on the other contemporary scaffolds architectures such as the tubular and cylindrical scaffolds show reduced cell proliferation in the bioreactor as compared to static conditions, at the same time point. Moreover, the rotating wall bioreactor does not alter the orientation or the phenotype of the Schwann cells on the aligned nanofiber containing scaffolds, wherein, the cells remain aligned along the length of the scaffolds. Therefore, these open structured spiral scaffolds pre-cultured with Schwann cells, in bioreactors could potentially shorten the time needed for grafts for peripheral nerve regeneration.
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影响因子：
2.1
作者：
Hadlock, TA;Sundback, CA;Cheney, ML
通讯作者：
Cheney, ML
影响因子：
14
作者：
Chew, Sing Ylan;Mi, Ruifa;Leong, Kam W.
通讯作者：
Leong, Kam W.
聚吡咯合成技术的生物相容性影响。
DOI：
10.1088/1748-6041/3/3/034124
发表时间：
2008-09
期刊：
Biomedical materials (Bristol, England)
影响因子：
--
作者：
Fonner JM;Forciniti L;Nguyen H;Byrne JD;Kou YF;Syeda-Nawaz J;Schmidt CE
通讯作者：
Schmidt CE
影响因子：
14
作者：
Liao, HH;Andersson, AS;Thomsen, P
通讯作者：
Thomsen, P
DOI：
10.1016/j.msec.2008.09.029
发表时间：
2009-05-05
期刊：
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
影响因子：
--
作者：
Ohyabu, Yoshimi;Tanaka, Junzo;Uemura, Toshimasa
通讯作者：
Uemura, Toshimasa