Pressed Lateral Flow Assay Strips for Flow Delay-Induced Signal Enhancement in Lateral Flow Assay Strips.
Pressed Lateral Flow Assay Strips for Flow Delay-Induced Signal Enhancement in Lateral Flow Assay Strips.
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DOI：
10.1007/s13206-022-00085-w
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发表时间：
2022
期刊：
影响因子：
4.3
通讯作者：
Shin, Joong Ho
中科院分区：
文献类型：
--
作者：
Park, Se Been;Shin, Joong Ho
本文提出了侧流检测（LFA）条的信号强度可以通过按压条的顶部来增加，从而有效地降低其流速。降低的流速允许更多的时间用于抗原-抗体相互作用的发生，导致信号强度增加和检测限提高。为了评估压制LFA（pLFA）条的潜力，检测在磷酸盐缓冲盐水（PBS）和血清中稀释的C-反应蛋白（CRP），提供信号增强和较低的检测限。此外，为了表明压力诱导的流动延迟引起的信号增强适用于现有的LFA产品，按压市售的COVID-19抗原测试条，观察到信号增强。最后，我们表明，通过在制造的产品顶部施加压力，COVID-19 LFA试剂盒的信号强度最多可以增加约两倍。这项研究表明，压制的LFA条可用于减少将模糊信号确定为假阴性结果的机会，并可能提高检测灵敏度。在线版本包含补充材料，可通过10.1007/s13206-022-00085-w获得。
This paper proposes that the signal intensity of a lateral flow assay (LFA) strip can be increased by pressing the top of the strip, effectively reducing its flow rate. The reduced flow rate allows more time for antigen–antibody interactions to occur, resulting in increased signal intensity and an improved detection limit. To assess the potential of the pressed LFA (pLFA) strip, C-reactive protein (CRP) diluted in phosphate-buffered saline (PBS) and serum is detected, affording signal enhancement and a lowered limit of detection. Additionally, to show that the signal enhancement by pressure-induced flow delay applies to existing LFA products, commercially available COVID-19 antigen test strips are pressed, and signal enhancement is observed. Lastly, we show that the signal intensity of COVID-19 LFA kits can be increased by approximately two-fold at maximum by applying pressure on top of the manufactured product. This study suggests that pressed LFA strips can be used to reduce the chances of determining ambiguous signals as false-negative results and can potentially improve the detection sensitivity. The online version contains supplementary material available at 10.1007/s13206-022-00085-w.
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DOI：
10.1016/j.snb.2020.129196
发表时间：
2021-02-15
期刊：
Sensors and actuators. B, Chemical
影响因子：
--
作者：
Liu H;Dai E;Xiao R;Zhou Z;Zhang M;Bai Z;Shao Y;Qi K;Tu J;Wang C;Wang S
通讯作者：
Wang S
影响因子：
8.8
作者：
Abdelazeem Hassan, Abdelrahim Hussein;Francisco Bergua, Jose;Mekoci, Arben
通讯作者：
Mekoci, Arben
影响因子：
12.6
作者：
Tang, Ye;Gao, Hui;Generelli, Silvia
通讯作者：
Generelli, Silvia
影响因子：
12.6
作者：
Sena-Torralba, Amadeo;Duy Ba Ngo;Merkoci, Arben
通讯作者：
Merkoci, Arben
影响因子：
6.1
作者：
Parolo, Claudio;Medina-Sanchez, Mariana;Merkoci, Arben
通讯作者：
Merkoci, Arben