Integrated Metabolomics and Transcriptome Analysis of Flavonoid Biosynthesis in Safflower (Carthamus tinctorius L.) With Different Colors.
Integrated Metabolomics and Transcriptome Analysis of Flavonoid Biosynthesis in Safflower (Carthamus tinctorius L.) With Different Colors.
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不同颜色红花中类黄酮生物合成的综合代谢组学和转录组分析
DOI：
10.3389/fpls.2021.712038
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发表时间：
2021
影响因子：
5.6
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Wang R;Ren C;Dong S;Chen C;Xian B;Wu Q;Wang J;Pei J;Chen J
红花广泛用于染料和传统医药，而C-葡糖基喹诺酮类化合物是红花红色的主要代谢物种。各种红花品种都有不同颜色的花。然而，花色不同的红花品种之间的代谢和转录差异以及参与C-葡萄糖基喹诺查尔酮生物合成的基因在很大程度上是未知的。为了对这一问题提供见解，我们对红花中不同颜色的花(白色-W、黄色-Y、浅红-LR和深红-DR)的类黄酮生物合成进行了综合代谢组学和转录组分析。代谢分析结果表明，不同颜色红花的总黄酮代谢产物有较大差异。在Y和W中检测到较多的类黄酮类代谢物种，而在W中未检测到C-葡萄糖基喹诺酮类化合物，C-葡萄糖基喹诺酮类化合物的含量随着颜色的增加而增加。转录分析表明，在W中，大多数注释的类黄酮生物合成基因都显着增加，与类黄酮生物合成相关的基因表达随着颜色的增加而降低。我们分析了与C-葡萄糖基喹诺酮类化合物相关的候选基因，结合代谢和转录分析表明，查尔酮合成酶(CHS)基因的差异表达是不同颜色红花类黄酮种类和含量差异的主要原因之一。结合表达谱分析，这些结果表明HH_035319、HH_032689和HH_018025可能参与了C-葡萄糖基喹诺酮类药物的生物合成。此外，我们还发现在茉莉酸甲酯(MeJA)处理后，它们的表达显著增加。因此，HH_035319、HH_032689和HH_018025可能参与了C-葡萄糖基喹诺查尔酮的生物合成，最终导致红花呈红色。
Safflower is widely used in dying and in traditional medicine, and C-glucosylquinochalcones are the main metabolic species in the red color of safflower. Various safflower cultivars have flowers with different colors. However, the metabolic and transcriptional differences among safflower cultivars with different-colored flowers and the genes participating in C-glucosylquinochalcone biosynthesis are largely unknown. To provide insights on this issue, we performed integrated metabolomics and transcriptome analyses on the flavonoid biosynthesis of flowers of different colors in safflower (white-W, yellow-Y, light red-LR, and deep red-DR). The metabolic analysis showed that flavonoid metabolites showed great differences among the different colors of safflower. More flavonoid metabolic species were detected in Y and W, while C-glucosylquinochalcones were not detected in W. The content of C-glucosylquinochalcones increased with increasing color. Transcriptional analysis showed that most of the annotated flavonoid biosynthesis genes were significantly increased in W. The expression of genes related to flavonoid biosynthesis decreased with increasing color. We analyzed the candidate genes associated with C-glucosylquinochalcones, and an integration of the metabolic and transcriptional analyses indicated that the differential expression of the chalcone synthase (CHS) gene is one of the main reasons for the difference in flavonoid species and content among the different colors of safflower. Combined with the expression pattern analysis, these results indicated that HH_035319, HH_032689, and HH_018025 are likely involved in C-glucosylquinochalcones biosynthesis. In addition, we found that their expression showed greatly increased after the methyl jasmonate (MeJA) treatment. Therefore, HH_035319, HH_032689, and HH_018025 might participate in C-glucosylquinochalcone biosynthesis, which ultimately leads to the red color in safflower.
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影响因子：
5.6
作者：
Jin X;Huang H;Wang L;Sun Y;Dai S
通讯作者：
Dai S
影响因子：
9.5
作者：
Moreno-Risueno MA;Busch W;Benfey PN
通讯作者：
Benfey PN
无花果（Ficus carica L.）果实颜色的调节：类黄酮生物合成途径的代谢组学和转录组学分析
影响因子：
5.6
作者：
Wang Z;Cui Y;Vainstein A;Chen S;Ma H
通讯作者：
Ma H
MeJA 处理下红花 (Carthamustinctorius L.) 类黄酮生物合成的综合代谢组学和转录组分析
影响因子：
5.3
作者：
Chen, Jiang;Wang, Jie;Pei, Jin
通讯作者：
Pei, Jin
影响因子：
8.8
作者：
De Paepe, Ellen;Wauters, Jella;Vanhaecke, Lynn
通讯作者：
Vanhaecke, Lynn