Structure and expression of the oncogene c-myc in fresh tumor material from patients with hematopoietic malignancies-猫眼课题宝

造血系统恶性肿瘤患者新鲜肿瘤材料中癌基因 c-myc 的结构和表达

DOI：

发表时间：

1984

期刊：

Molecular and Cellular Biology

影响因子：

5.3

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

P. Rothberg;M. Erisman;R. Diehl;U. Rovigatti;S. Astrin

c-myc是与急性禽类逆转录病毒MC 29的转化序列同源的细胞基因。克隆了人c-myc基因，并用于研究c-myc基因的结构及其在多种造血系统恶性肿瘤中的表达。在对106名患者的仔细研究中，发现c-myc RNA在17名白血病患者和5名淋巴瘤患者的肿瘤细胞中表达水平升高。c-myc基因被发现在两个淋巴瘤，非洲伯基特淋巴瘤和非霍奇金淋巴瘤在白血病期重排。Burkitt重排涉及在c-myc 5'编码区上游插入新的DNA序列，可能取代了正常的c-myc转录启动子。其他104名患者，包括20名myc表达升高的患者，均未表现出任何涉及c-myc基因的遗传重排证据。我们的研究结果表明，有一个子集的造血系统恶性肿瘤的特点是c-myc的表达升高。在大多数情况下，这种升高的表达不是由于c-myc基因座的明显遗传变化（重排、扩增），也不是由于该基因附近的染色体易位。

c-myc is the cellular gene homologous to the transforming sequence of MC29, an acute avian retrovirus. The human c-myc gene was cloned and used to study the structure and expression of c-myc in a variety of human hematopoietic malignancies. In a careful study of 106 patients, c-myc RNA was found to be expressed at elevated levels in tumor cells of 17 leukemia patients and five lymphoma patients. The c-myc gene was found to be rearranged in two lymphomas, an African Burkitt's lymphoma and a non-Hodgkins lymphoma in leukemic phase. The Burkitt's rearrangement involved the insertion of new DNA sequences upstream from the c-myc 5' coding region, presumably replacing the normal c-myc transcriptional promoter. None of the other 104 patients, including 20 with elevated myc expression, exhibited any evidence of a genetic rearrangement involving the c-myc gene. Our results show that there is a subset of hematopoietic malignancies characterized by elevated expression of c-myc. This elevated expression in most cases is not due to obvious genetic changes (rearrangement, amplification) at the c-myc locus nor to chromosomal translocations in the vicinity of this gene.