Sequence and expression analysis of potential nonstructural proteins of 4.9, 4.8, 12.7, and 9.5 kDa encoded between the spike and membrane protein genes of the bovine coronavirus.
Sequence and expression analysis of potential nonstructural proteins of 4.9, 4.8, 12.7, and 9.5 kDa encoded between the spike and membrane protein genes of the bovine coronavirus.
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DOI：
10.1016/0042-6822(90)90513-q
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发表时间：
1990-08
期刊：
影响因子：
3.7
通讯作者：
Brian DA
中科院分区：
文献类型：
--
作者：
Abraham S;Kienzle TE;Lapps WE;Brian DA
牛冠状病毒（BCV）基因组中刺突蛋白和膜蛋白基因之间的核苷酸序列通过对基因组的cDNA克隆进行测序来确定，并鉴定了按该顺序可能编码4.9、4.8、12.7和9.5 kDa蛋白的开放阅读框。4.9-和4.8-kDa蛋白似乎是11-kDa蛋白的遗迹，对于该蛋白，基因中心部分的单核苷酸缺失事件产生了终止密码子。共有CYAAAC序列位于4.9-、12.7-和9.5-kDa ORF之前，并预测转录将从这些位点中的每一个开始。使用序列特异性探针和oligo（dT）选择的RNA的北方分析证明了预测的转录物的产生，并且这些转录物对应于mRNA 4、5和5-1。BCV mRNA 4似乎是小鼠肝炎病毒（MHV）mRNA 4的对应物，在MHV JHM株中，MHV mRNA 4编码推定的15.2 kDa非结构蛋白。BCV mRNA 5和5-1似乎分别用于合成12.7和9.5 kDa蛋白，这表明表达模式与MHV所利用的表达模式显著不同。MHV从单个mRNA 5中产生12.7-和9.5-kDa蛋白质的同源物。体外翻译分析表明，与MHV对应物不同，BCV9.5-kDa蛋白质在下游翻译起始中的作用很差。因此，从BCV和MHV之间的比较中，我们发现了CYAAAC序列在调节冠状病毒转录中的重要性的进化证据。
The nucleotide sequence between the spike and membrane protein genes in the bovine coronavirus (BCV) genome was determined by sequencing cDNA clones of the genome, and open reading frames potentially encoding proteins of 4.9, 4.8, 12.7, and 9.5 kDa, in that order, were identified. The 4.9- and 4.8-kDa proteins appear to be vestiges of an 11-kDa protein for which a single nucleotide deletion event in the central part of the gene gave rise to a stop codon. The consensus CYAAAC sequence precedes the 4.9-, 12.7-, and 9.5-kDa ORFs and predicts that transcription will start from each of these sites. Northern analyses using sequence-specific probes and oligo(dT)-selected RNA demonstrated that the predicted transcripts are made, and that these correspond to mRNAs 4, 5, and 5-1. BCV mRNA 4 appears to be a counterpart to mouse hepatitis virus (MHV) mRNA 4 which, in the MHV JHM strain, encodes the putative 15.2-kDa nonstructural protein. BCV mRNAs 5 and 5-1 appear to be used for the synthesis of the 12.7- and 9.5-kDa proteins, respectively, which demonstrates a pattern of expression strikingly different from that utilized by MHV. MHV makes its homologs of the 12.7- and 9.5-kDa proteins from the single mRNA 5. In vitro translation analyses demonstrated that the BCV 9.5-kDa protein, unlike its MHV counterpart, is poorly made from downstream initiation of translation. Thus, from a comparison between BCV and MHV we find evolutionary evidence for the importance of the CYAAAC sequence in regulating coronavirus transcription.
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DOI：
10.1083/jcb.108.2.229
发表时间：
1989-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kozak M
通讯作者：
Kozak M
影响因子：
3.7
作者：
Budzilowicz CJ;Weiss SR
通讯作者：
Weiss SR
影响因子：
3.8
作者：
BOURSNELL, MEG;BINNS, MM;BROWN, TDK
通讯作者：
BROWN, TDK
影响因子：
3.7
作者：
PACHUK, CJ;BREDENBEEK, PJ;WEISS, SR
通讯作者：
WEISS, SR
影响因子：
3.7
作者：
Kapke PA;Brian DA
通讯作者：
Brian DA