Transcriptional Profiling of C. albicans in a Two Species Biofilm with Rothia dentocariosa.
Transcriptional Profiling of C. albicans in a Two Species Biofilm with Rothia dentocariosa.
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DOI：
10.3389/fcimb.2017.00311
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发表时间：
2017
影响因子：
5.7
通讯作者：
Chaffin WL
中科院分区：
文献类型：
--
作者：
Uppuluri P;Busscher HJ;Chakladar J;van der Mei HC;Chaffin WL
硅橡胶发音假体上的生物膜是这些设备频繁失效和更换的主要原因。细菌和酵母菌株的存在已被认为是至关重要的语音假体生物膜的发展。包括白色念珠菌和龋齿罗氏菌在内的多种微生物生物膜是发音假体失败的主要原因。在硅橡胶上开发了包含这两种生物体的体外生物膜，硅橡胶是用于格罗宁根按钮发音假体的材料。我们发现这种生物膜环境不利于C.白色念珠菌生长或分化。对C.白色念珠菌生物膜细胞与R. dentocariosa揭示了与细胞周期进程和菌丝发育相关的功能基因被抑制> 2倍。与C.白色念珠菌单种生物膜，即使在连续营养流的条件下发展。在此条件下，R. dentocariosa也显著抑制C.白色念珠菌生物膜扩散。R.粘附素Als3介导龋齿对念珠菌菌丝的粘附作用。
Biofilms on silicone rubber voice prostheses are the major cause for frequent failure and replacement of these devices. The presence of both bacterial and yeast strains has been suggested to be crucial for the development of voice prosthetic biofilms. Polymicrobial biofilms that include Candida albicans and Rothia dentocariosa are the leading cause of voice prosthesis failure. An in vitro biofilm comprising these two organisms was developed on silicone rubber, a material used for Groningen button voice prosthesis. We found that this biofilm environment was not conducive for C. albicans growth or differentiation. Global transcriptional analyses of C. albicans biofilm cells grown with R. dentocariosa revealed that genes with functions related to cell cycle progression and hyphal development were repressed >2-fold. The mixed species biofilms were more compact and less robust compared to C. albicans mono-species biofilms, even when developed under conditions of continuous nutrient flow. Under these conditions R. dentocariosa also significantly inhibited C. albicans biofilm dispersal. Preferential adherence of R. dentocariosa to candidal hyphae was mediated by the adhesin Als3.
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影响因子：
2.6
作者：
Elving, GJ;van der Mei, HC;Albers, FWJ
通讯作者：
Albers, FWJ
影响因子：
3.1
作者：
Diaz, Patricia I.;Xie, Zhihong;Dongari-Bagtzoglou, Anna
通讯作者：
Dongari-Bagtzoglou, Anna
影响因子：
--
作者：
Peters BM;Jabra-Rizk MA;Scheper MA;Leid JG;Costerton JW;Shirtliff ME
通讯作者：
Shirtliff ME
影响因子：
3.6
作者：
Hogan, DA;Vik, Å;Kolter, R
通讯作者：
Kolter, R
影响因子：
56.9
作者：
Hogan, DA;Kolter, R
通讯作者：
Kolter, R