Ectopic expression of VpALDH2B4, a novel aldehyde dehydrogenase gene from Chinese wild grapevine (Vitis pseudoreticulata), enhances resistance to mildew pathogens and salt stress in Arabidopsis
Ectopic expression of VpALDH2B4, a novel aldehyde dehydrogenase gene from Chinese wild grapevine (Vitis pseudoreticulata), enhances resistance to mildew pathogens and salt stress in Arabidopsis
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来自中国野生葡萄（Vitis pseudoreticulata）的一种新型醛脱氢酶基因 VpALDH2B4 的异位表达可增强拟南芥对霉菌病原体和盐胁迫的抵抗力
DOI：
10.1007/s00425-012-1624-z
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发表时间：
2012-03
期刊：
影响因子：
4.3
通讯作者：
Wang, Yuejin
中科院分区：
文献类型：
--
作者：
Wen, Yingqiang;Wang, Xiping;Xiao, Shunyuan;Wang, Yuejin
乙醛脱氢酶(ALDHs)催化广泛的反应性醛不可逆地氧化成相应的羧酸。虽然已经从不同的生物体和植物的不同生长阶段对这些蛋白进行了研究，但还没有研究它们在病原体侵染中的潜在作用。在这里，我们从中国野生葡萄品种白河-35-1中分离到一个病原菌诱导的ALDH基因(VpALDH_2B4)，并对其进行了功能鉴定。当VpALDH2B4在拟南芥叶片中瞬时表达时，发现其定位于线粒体。表达GST-VpALDH2B4的大肠杆菌在体外具有ALDH活性，并能利用丙二醛、乙醛和甘油醛作为底物。VpALDH_2B4在拟南芥中的过表达导致了类似超敏反应的细胞死亡，增强了对霜霉病和白粉病的抗性，这可能是通过SA信号途径实现的。相同的拟南芥转基因植株也表现出对盐胁迫的耐受性增强，伴随着丙二醛的积累减少和胁迫响应超氧化物歧化酶活性的上调。综上所述，我们的结果表明，VpALDH2B4及其同源基因可能参与了植物对生物营养病原体和高盐胁迫的响应。
Aldehyde dehydrogenases (ALDHs) catalyze the irreversible oxidation of a broad spectrum of reactive aldehydes to their corresponding carboxylic acids. Although the proteins have been studied from various organisms and at different growth stages in plants, their potential roles in pathogen infection have not been examined. Here we isolated and functionally characterized a pathogen-inducible ALDH gene (VpALDH2B4) from Chinese wild grapevine Vitis pseudoreticulata accession Baihe-35-1. When transiently expressed in Arabidopsis leaves, VpALDH2B4 was found to be localized in mitochondria. Escherichia coli expressed GST-VpALDH2B4 exhibited ALDH activity in vitro and was capable of utilizing malondialdehyde (MDA), acetaldehyde and glyceraldehydes as its substrate. Over-expression of VpALDH2B4 in Arabidopsis resulted in hypersensitive response-like cell death, enhanced resistance to downy mildew and powdery mildew presumably via the SA-signaling pathway. The same Arabidopsis transgenic plants also showed enhanced tolerance to salt stress, which is accompanied by less MDA accumulation and upregulation of the stress-responsive superoxide dismutase activity. Taken together, our results suggest that VpALDH2B4 and perhaps its orthologous genes may be involved in responses of plants to stresses imposed by both biotrophic pathogens and high salinity conditions.
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