Transcriptional responses of wheat and the cereal cyst nematode Heterodera avenae during their early contact stage.
Transcriptional responses of wheat and the cereal cyst nematode Heterodera avenae during their early contact stage.
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小麦和谷类胞囊线虫燕麦异皮线虫早期接触阶段的转录反应
DOI：
10.1038/s41598-017-14047-y
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发表时间：
2017-11-03
影响因子：
4.6
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Chen C;Cui L;Chen Y;Zhang H;Liu P;Wu P;Qiu D;Zou J;Yang D;Yang L;Liu H;Zhou Y;Li H
小麦包囊线虫（Heterodera avenae）被吸引并聚集在小麦根部周围引发感染，但小麦与线虫之间的相互作用尚不完全清楚。采用mRNA序列分析方法，研究了小麦和绿僵菌在接触早期的转录响应；采用实时荧光定量PCR技术对一定数量的差异表达基因（deg）进行验证。固定寄主小麦根系只有93个deg（27个上调，66个下调），而移动寄主小麦根系对879个deg（867个上调，12个下调）的反应更为活跃。其中，许多小麦deg（大多下调）参与了生物胁迫途径，而线虫deg中一些可能的效应基因上调。一种推测的H. avenae几丁质酶样效应基因能够抑制bax引发的烟叶程序性细胞死亡。实验结果表明，在被寄生的接触阶段，线虫的反应比小麦更积极。寄生物的反应主要涉及至少一种抗植物防御效应基因的上调，而寄主的反应主要涉及某些防御相关基因的下调。
Cereal cyst nematode (Heterodera avenae) is attracted to and aggregated around wheat roots to initiate infection, but this interaction between wheat and the nematode is not fully understood. The transcriptional responses of both wheat and H. avenae were examined during their early contact stage by mRNA sequencing analysis; certain numbers of the differentially expressed genes (DEGs) were validated using quantitative real-time PCR. The immobile host wheat root only had 93 DEGs (27 up-regulated and 66 down-regulated), while the mobile plant parasitic nematode reacted much more actively with 879 DEGs (867 up-regulated and 12 down-regulated). Among them, a number of wheat DEGs (mostly down-regulated) were involved in biotic stress pathways, while several putative effector genes were up-regulated in the nematode DEGs. One putative chitinase-like effector gene of H. avenae was able to suppress BAX-triggered programmed cell death in Nicotiana benthamiana. Results of these experiments demonstrated that nematode responded more actively than wheat during the contact stage of parasitism. The parasite’s responses mainly involved up-regulation of genes including at least one anti-plant-defence effector gene, whereas the host responses mainly involved down-regulation of certain defence-related genes.
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影响因子：
7.2
作者：
Barcala, Marta;Garcia, Alejandra;Escobar, Carolina
通讯作者：
Escobar, Carolina
影响因子：
1.5
作者：
Gang SS;Hallem EA
通讯作者：
Hallem EA
影响因子：
12.3
作者：
Cotton JA;Lilley CJ;Jones LM;Kikuchi T;Reid AJ;Thorpe P;Tsai IJ;Beasley H;Blok V;Cock PJ;Eves-van den Akker S;Holroyd N;Hunt M;Mantelin S;Naghra H;Pain A;Palomares-Rius JE;Zarowiecki M;Berriman M;Jones JT;Urwin PE
通讯作者：
Urwin PE
影响因子：
14.9
作者：
Edgar, R;Domrachev, M;Lash, AE
通讯作者：
Lash, AE
影响因子：
3.7
作者：
Fosu-Nyarko J;Nicol P;Naz F;Gill R;Jones MG
通讯作者：
Jones MG