Exercise reverses the effects of early life stress on orexin cell reactivity in male but not female rats.
Exercise reverses the effects of early life stress on orexin cell reactivity in male but not female rats.
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DOI：
10.3389/fnbeh.2014.00244
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发表时间：
2014
影响因子：
3
通讯作者：
Dayas CV
中科院分区：
文献类型：
--
作者：
James MH;Campbell EJ;Walker FR;Smith DW;Richardson HN;Hodgson DM;Dayas CV
早期生活压力（ELS）是抑郁症等情绪障碍发展的已知前因。食欲素神经元在应激时驱动唤醒和动机行为。我们测试了ELS改变食欲素系统功能并导致成年期应激诱导行为表型改变的假设。我们还调查了在青少年发育过程中自愿锻炼是否可以逆转ELS引起的变化。雄性和雌性Wistar大鼠在出生后第2-14天（PND）受到母体分离应激。在青春期后期（1小时/天，PND 40 -70），一部分动物可以使用转轮。成年后，大鼠暴露于束缚应激，然后在开放场（OF）和高架十字迷宫（ESTA）上进行测试。对脑进行Fos蛋白和食欲素或酪氨酸羟化酶免疫组织化学处理。束缚应激刺激Fos蛋白在穹窿周围区食欲素细胞，下丘脑室旁核，丘脑脑室旁核的表达，但这种神经元的反应在暴露于ELS的雄性和雌性大鼠中被抑制。ELS还减少了在OF中的探索，而不影响探索行为。这些神经和行为变化与抑郁样表型一致。青少年运动逆转了ELS男性的食欲素和行为缺陷。运动对女性没有保护作用，尽管这可能是由于跑步行为的性别差异。我们的研究结果强调了食欲素系统的内在可塑性，这种特性可能导致病理性的重新连接状态，但也可以使用非药物方法进行治疗。我们还强调需要更好地了解食欲素回路和压力相关病理学的性别特异性变化。
Early life stress (ELS) is a known antecedent for the development of mood disorders such as depression. Orexin neurons drive arousal and motivated behaviors in response to stress. We tested the hypothesis that ELS alters orexin system function and leads to an altered stress-induced behavioral phenotype in adulthood. We also investigated if voluntary exercise during adolescent development could reverse the ELS-induced changes. Male and female Wistar rats were subjected to maternal separation stress on postnatal days (PND) 2-14. A subset of animals was given access to running wheels in late adolescence (1hr/day, PND40-70). In adulthood, rats were exposed to restraint stress and then tested on the open field (OF) and elevated plus maze (EPM). Brains were processed for Fos-protein and orexin or tyrosine hydroxylase immunohistochemistry. Restraint stress stimulated Fos-protein expression in perifornical area orexin cells, the paraventricular hypothalamic nucleus, and paraventricular thalamic nuclei, but this neuronal response was dampened in male and female rats exposed to ELS. ELS also reduced exploration in the OF, without affecting EPM behavior. These neural and behavioral changes are consistent with a depressive-like phenotype. Adolescent exercise reversed the orexin and behavioral deficits in ELS males. Exercise was not protective in females, although this may be due to sex differences in running behavior. Our findings highlight the inherent plasticity of the orexin system—a trait that may lead to a state of pathological rewiring but could also be treated using non-pharmacological approaches. We also highlight a need to better understand the sex-specific changes in orexin circuits and stress-related pathology.
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影响因子：
4.7
作者：
Brocardo, Patricia S.;Boehme, Fanny;Christie, Brian R.
通讯作者：
Christie, Brian R.
影响因子：
7.6
作者：
Caldji, C;Francis, D;Meaney, MJ
通讯作者：
Meaney, MJ
丘脑室旁核微量注射食欲素引起的情绪行为变化
影响因子：
3.6
作者：
Li, Yonghui;Li, Sa;Kirouac, Gilbert J.
通讯作者：
Kirouac, Gilbert J.
影响因子：
3.4
作者：
Dayas, CV;Buller, KM;Day, TA
通讯作者：
Day, TA
DOI：
10.1006/bbrc.2000.2412
发表时间：
2000-04-02
影响因子：
3.1
作者：
Ida, T;Nakahara, K;Murakami, N
通讯作者：
Murakami, N