Differences in host breed and diet influence colonization by Campylobacter jejuni and induction of local immune responses in chicken.
Differences in host breed and diet influence colonization by Campylobacter jejuni and induction of local immune responses in chicken.
复制标题
DOI：
10.1186/s13099-016-0133-1
复制
发表时间：
2016
期刊：
影响因子：
4.2
通讯作者：
Rautenschlein S
中科院分区：
文献类型：
--
作者：
Han Z;Willer T;Pielsticker C;Gerzova L;Rychlik I;Rautenschlein S
鸡被认为是人类弯曲杆菌病的主要宿主。关于空肠弯曲菌与鸡之间的相互作用，人们知之甚少。这种相互作用可能受到免疫系统成熟阶段、肠道微生物区系组成的发展以及包括品种和饮食在内的其他因素的影响。本研究的目的是研究品种和日粮对空肠弯曲菌在鸡体内定植和宿主免疫反应的影响。用104个空肠弯曲菌集落形成单位(CFU)或1个稀释液(Exp.1)或22(实验2)孵化后天数。我们比较了用商业肉鸡饲料(Bf)和蛋鸡饲料(Lf)饲养的肉鸡型(BT)和蛋鸡(LT)禽类的局部免疫细胞亚群、细胞因子表达水平和肠道微生物区系组成。在年龄较大的人群中观察到较低的定殖率，与品种和饮食无关。与饲喂bf组相比，饲喂bf组雏鸡空肠弯曲菌CFU数高于饲喂bf组。空肠弯曲菌接种对Bt雏鸡淋巴细胞数量和细胞因子表达水平有显著影响，与饲养策略无关(p>0.05)。在LT雏鸡中没有检测到这些影响，只有饲喂bf的LT雏鸡在接种空肠弯曲菌后7天时，IL-8的表达显著高于LT对照组雏鸡(P<0.05)。日粮对Bt和LT鸡肠道微生物区系组成的影响具有可比性，但与空肠弯曲菌接种相关的微生物区系组成的变化因品种而异。与LT和Bt相比，饮食和品种对空肠弯曲菌定殖率、免疫应答和微生物区系组成的影响程度不同。这些差异背后的机制必须进一步阐明。我们的结果表明，选择抗性更强的品种，结合适当的饲养策略，可能有助于降低未来商业家禽中弯曲杆菌的定植水平。
Chickens are regarded as the main reservoir for human campylobacteriosis. Little is known about the interaction between Campylobacter jejuni (C. jejuni) and chickens. This interaction may be influenced by the stage of maturation of the immune system, developing gut microbiota composition and other factors including breed and diet. Our aim was to investigate the impact of breed, and diet on C. jejuni colonization and host immune responses in chickens. Birds were inoculated with 104 colony forming units (CFU) of C. jejuni or diluent at one (Exp. 1) or 22 (Exp. 2) days post hatch. We compared local immune cell subpopulations, cytokine expression levels, and gut microbiota composition between broiler-type (BT) and layer-type (LT) birds fed with either commercial broiler feed (bf) or layer feed (lf). Lower colonization rates were observed in the older age group independent of breed and diet. Independent of breed, birds fed with bf showed higher CFU of C. jejuni compared to lf-fed groups. Campylobacter jejuni-inoculation had a significant effect on lymphocyte numbers and cytokine expression levels in BT birds independent of feeding strategy (p < 0.05). These effects were not detected in LT birds, only LT birds fed with bf showed a significant increase in IL-8-expression at 7 days post C. jejuni inoculation compared to LT-control birds (p < 0.05). Diet influenced gut microbiota composition in a comparable manner between BT and LT birds, but changes in microbiota composition associated with C. jejuni inoculation varied between breeds. Diet and breed influenced C. jejuni colonization, immune responses and microbiota composition to a different extent comparing between LT and BT birds. The mechanisms behind these differences have to be elucidated further. Our results suggest that selection for more resistant breeds in combination with adapted feeding strategies may help to reduce Campylobacter colonization levels in commercial poultry in the future.
登录
查看更多内容
DOI：
10.1016/j.ijfoodmicro.2009.01.033
发表时间：
2009-05-31
影响因子：
5.4
作者：
Bronzwaer, S.;Hugas, M.;Havelaar, A.
通讯作者：
Havelaar, A.
影响因子：
4.4
作者：
Chaloner, Gemma;Wigley, Paul;Williams, Nicola
通讯作者：
Williams, Nicola
影响因子：
2
作者：
Arsi, K.;Donoghue, A. M.;Donoghue, D. J.
通讯作者：
Donoghue, D. J.
影响因子：
1.4
作者：
Dhillon, AS;Shivaprasad, HL;Bandli, D
通讯作者：
Bandli, D
影响因子：
1.4
作者：
Knudsen, KN;Bang, DD;Madsen, M
通讯作者：
Madsen, M