Patterns of expression of class I antigens in the tissues of congenic strains of rat.

Patterns of expression of class I antigens in the tissues of congenic strains of rat.
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I类抗原在大鼠同源品系组织中的表达模式。

DOI:
10.1089/hyb.1985.4.27
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发表时间:
1985
期刊:
影响因子:
--
通讯作者:
Iannaccone,PM
Iannaccone,PM
中科院分区:
--
文献类型:
--
作者:
Weinberg,WC;Deamant,FD;Iannaccone,PM

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对大鼠肺、脾、肾、心、肝和脑组织中I类主要组织相容性(MHC)抗原的表达进行了比较研究。将抗RT 1A α决定簇的单克隆抗体(MAbs)与125 I偶联并应用于冷冻切片。结果放射自显影显示淋巴组织、支气管和肺泡上皮以及肺内皮中的抗原反应性。肺和脾的淋巴样组织在比这些器官的上皮成分所需的更短的放射自显影曝光后显示出抗原。肾脏在肾小球上标记较重,但在肾小管上皮上标记较弱。心肌毛细血管内皮细胞的RT 1A抗原含量高于肌束。在肝脏中,放射自显影切片显示窦状隙区域的高决定簇密度。脑切片显示可再现的低水平标记,血管结构除外。PVG-RT 1c和PVG-RT 1u动物的所有这些组织仅显示背景标记。
A comparative study of expression of class I major histocompatibility (MHC) antigens among lung, spleen, kidney, heart, liver, and brain tissues of the rat was performed. Monoclonal antibodies (MAbs) against RT1Aadeterminants were conjugated to125I and applied to frozen sections. Resulting autoradiograms showed antigen reactivity in lymphoid tissue, bronchial and alveolar epithelium, and endothelium of the lung. The Iymphoid tissue of the lung and spleen demonstrated the antigen after shorter autoradiographic exposures than was required for the epithelial components of these organs. The kidneys were heavily labeled over the glomeruli, but less intensely over the tubular epithelium. RT1A antigen content of capillary endothelium of the heart was demonstrable before that of the muscle bundles. In the liver, autoradiographic sections revealed high determinant density in sinusoidal regions. Brain sections show reproducibly low levels of labeling, with the exception of vascular structures. All of these tissues from PVG-RT1cand PVG-RT1uanimals show only background labeling.
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