Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda.
Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda.
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DOI：
10.1099/vir.0.014183-0
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发表时间：
2009-11
期刊：
影响因子：
--
通讯作者：
de Lamballerie X
中科院分区：
文献类型：
--
作者：
Cook S;Moureau G;Harbach RE;Mukwaya L;Goodger K;Ssenfuka F;Gould E;Holmes EC;de Lamballerie X
黄病毒属包含大约70种单链、阳性RNA病毒，它是研究媒介传播疾病进化的独特模型，因为它包括蚊媒传播、扁虱传播或没有已知媒介的病毒。理论工作和实地研究都表明，存在大量未被发现的黄病毒。最近，从波多黎各的一个自然蚊子种群中首次分离到细胞融合剂病毒(CFAV)，并在泰国的蚊子中发现了与CFAV相关的序列。CFAV曾于1975年从蚊子细胞系中分离出来，是唯一已知的仅限于昆虫的黄病毒，似乎只在昆虫细胞中复制。卡米提河病毒(KRV)是“纯昆虫”组的第二个成员，于2003年从肯尼亚蚊子中分离出来。2007年首次从日本分离到第三个暂定的“纯昆虫”组成员--库蚊黄病毒(CxFV)，随后从美洲报告了更多的病毒株。我们报道了在乌干达恩德培发现、分离和鉴定了两种新的“纯昆虫”黄病毒：一种新的谱系暂定名为Nakiwogo病毒(NAKV)和一种新的CxFV株。利用从该地区采集的凭证样本生成的参考分子系统发育树，对分离到的这些菌株的个体蚊子进行了回顾性鉴定，分别是非洲曼森纳蚊子和致倦库蚊。据我们所知，这是从曼索尼亚物种中首次分离到一种新的昆虫黄病毒，也是从非洲首次分离到一株CxFV。
The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies suggest the existence of a large number of undiscovered flaviviruses. Recently, the first isolation of cell fusing agent virus (CFAV) was reported from a natural mosquito population in Puerto Rico, and sequences related to CFAV have been discovered in mosquitoes from Thailand. CFAV had previously been isolated from a mosquito cell line in 1975 and represented the only known ‘insect-only’ flavivirus, appearing to replicate in insect cells alone. A second member of the ‘insect-only’ group, Kamiti River virus (KRV), was isolated from Kenyan mosquitoes in 2003. A third tentative member of the ‘insect-only’ group, Culex flavivirus (CxFV), was first isolated in 2007 from Japan and further strains have subsequently been reported from the Americas. We report the discovery, isolation and characterization of two novel ‘insect-only’ flaviviruses from Entebbe, Uganda: a novel lineage tentatively designated Nakiwogo virus (NAKV) and a new strain of CxFV. The individual mosquitoes from which these strains were isolated, identified retrospectively by using a reference molecular phylogeny generated using voucher specimens from the region, were Mansonia africana nigerrima and Culex quinquefasciatus, respectively. This represents the first isolation, to our knowledge, of a novel insect-only flavivirus from a Mansonia species and the first isolation of a strain of CxFV from Africa.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
3.7
作者：
Kim, Dal Y.;Guzman, Hilda;Tesh, Robert B.
通讯作者：
Tesh, Robert B.
DOI：
10.4269/ajtmh.2009.80.85
发表时间：
2009-01-01
影响因子：
3.3
作者：
Farfan-Ale, Jose A.;Lorono-Pino, Maria A.;Blitvich, Bradley J.
通讯作者：
Blitvich, Bradley J.
影响因子：
3.7
作者：
Grard, Gilda;Moureau, Gregory;de Lamballerie, Xavier
通讯作者：
de Lamballerie, Xavier
影响因子：
2.7
作者：
Crabtree, MB;Sang, RC;Miller, BR
通讯作者：
Miller, BR