Insights into the origin of metazoan filopodia and microvilli.
Insights into the origin of metazoan filopodia and microvilli.
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DOI：
10.1093/molbev/mst110
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发表时间：
2013-09
影响因子：
10.7
通讯作者：
Ruiz-Trillo I
中科院分区：
文献类型：
--
作者：
Sebé-Pedrós A;Burkhardt P;Sánchez-Pons N;Fairclough SR;Lang BF;King N;Ruiz-Trillo I
丝状伪足是细胞内以肌动蛋白为基础的精细突起，用于环境感知和细胞运动，是后生动物细胞的重要细胞器。在这项研究中，我们重建了后生动物丝状伪足和微绒毛的起源。我们首先报告的进化组装的丝状伪足分子工具包，并表明，同源物的许多后生动物丝状伪足组件，包括肌成束蛋白和肌球蛋白X，已经存在于单细胞或殖民地的后生动物的祖先。此外，我们发现肌动蛋白交联蛋白fascin定位于丝状伪足样结构和微绒毛在choanoflagellate Salpingoeca rosetta。此外，同源的丝状足基因在全虫Capsaspora owczarzaki上调，相对于那些缺乏丝状足轴承细胞。因此，我们的研究结果表明，后生动物丝状伪足和微绒毛所必需的蛋白质在单细胞和殖民地的完整动物中是功能保守的，后生动物的最后共同祖先具有复杂和特定的丝状伪足机制。
Filopodia are fine actin-based cellular projections used for both environmental sensing and cell motility, and they are essential organelles for metazoan cells. In this study, we reconstruct the origin of metazoan filopodia and microvilli. We first report on the evolutionary assembly of the filopodial molecular toolkit and show that homologs of many metazoan filopodial components, including fascin and myosin X, were already present in the unicellular or colonial progenitors of metazoans. Furthermore, we find that the actin crosslinking protein fascin localizes to filopodia-like structures and microvilli in the choanoflagellate Salpingoeca rosetta. In addition, homologs of filopodial genes in the holozoan Capsaspora owczarzaki are upregulated in filopodia-bearing cells relative to those that lack them. Therefore, our findings suggest that proteins essential for metazoan filopodia and microvilli are functionally conserved in unicellular and colonial holozoans and that the last common ancestor of metazoans bore a complex and specific filopodial machinery.
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影响因子：
12.3
作者：
Fairclough SR;Chen Z;Kramer E;Zeng Q;Young S;Robertson HM;Begovic E;Richter DJ;Russ C;Westbrook MJ;Manning G;Lang BF;Haas B;Nusbaum C;King N
通讯作者：
King N
影响因子：
2.5
作者：
Cavalier-Smith, Thomas;Chao, Ema E.
通讯作者：
Chao, Ema E.
影响因子：
2.2
作者：
Karpov, SA;Leadbeater, BSC
通讯作者：
Leadbeater, BSC
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
21.3
作者：
Berg, JS;Cheney, RE
通讯作者：
Cheney, RE