Crystalline two-dimensional DNA-origami arrays.
Crystalline two-dimensional DNA-origami arrays.
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DOI：
10.1002/anie.201005911
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发表时间：
2011-01-03
影响因子：
16.6
通讯作者：
Seeman, Nadrian C.
中科院分区：
文献类型：
--
作者：
Liu, Wenyan;Zhong, Hong;Wang, Risheng;Seeman, Nadrian C.
纳米技术旨在以尽可能高的精度和控制来组织物质。这种控制将导致纳米电子学，纳米机器人，可编程化学合成，支架晶体和纳米系统响应其环境。结构DNA纳米技术1是实现这一目标的最有力途径之一。它将强大的分支DNA种类与粘性末端可编程性中固有的亲和力和结构2的控制相结合。结构DNA纳米技术的成功包括物体的形成，3 2D晶体，4 3D晶体，5纳米机械装置，6和这些物种的各种组合（例如，参考文献7）。DNA origami 8可以说是在2D DNA表面上产生大的可寻址区域的最有效方法。该方法需要将长单链（通常为M13单链形式，7249个核苷酸）与约250个钉合链组合以限定其形状和图案。在像素化估计为约6 nm的情况下，8可以在约10，000 nm 2的区域中的可定义形状内构建具有约100个可寻址点的图案。许多研究人员试图通过形成单个折纸瓦片的晶体来增加2D折纸单元的有用尺寸，但没有成功。在这里，我们报告了折纸瓦片的2D结晶，以产生边缘尺寸为2-3微米的2D阵列。这个尺寸可能足够大，可以将自下而上的图案化方法与自上而下的方法联系起来，晶体阵列是传播图案和其他物质分布的方便方法，因此多个副本可以自组织成大的周期性或非周期性系统。DNA是一种特别强大的组织系统，因为它可以用粘性末端连接结构基序，因此沃森-克里克互补相互作用可以被编程以建立分子间的接触。1 DNA双交叉（DX）基序是小瓦片（~ 4 nm×~ 16 nm）的例子，它们被编程以产生2D晶体; 4当不止一个瓦片构成晶体学重复时，这些瓦片通常包含图案形成特征。这些图案包含两个平行的双螺旋，通过交叉保持在一起;第二维来自连接给定瓦片的一个螺旋到相邻瓦片的另一个螺旋，如图1a所示。除了图中所示的周期性图案外，这种分子间组织形式还被用于
Nanotechnology aims to organize matter with the highest possible accuracy and control. Such control will lead to nanoelectronics, nanorobotics, programmable chemical synthesis, scaffolded crystals, and nanoscale systems responsive to their environments. Structural DNA nanotechnology1 is one of the most powerful routes to this goal. It combines robust branched DNA species with the control of affinity and structure2 inherent in the programmability of sticky ends. The successes of structural DNA nanotechnology include the formation of objects, 3 2D crystals, 4 3D crystals, 5 nanomechanical devices, 6 and various combinations of these species (eg, ref. 7). DNA origami8 is arguably the most effective way of producing a large addressable area on a 2D DNA surface. This method entails the combination of a long single strand (typically M13 single-stranded form, 7249 nucleotides) with about~ 250 staple strands to define its shape and patterning. With a pixilation estimated at about 6 nm, 8 it is possible to build patterns with about 100 addressable points within a definable shape in an area of about 10,000 nm2. Many investigators have sought unsuccessfully to increase the useful size of 2D origami units by forming crystals of individual origami tiles. 0 Here, we report the 2D crystallization of origami tiles to yield a 2D array with dimensions 2–3 microns on an edge. This size is likely to be large enough to connect bottom-up methods of patterning with top-down approaches.Crystalline arrays are a convenient way to propagate patterns and other distributions of matter, so that multiple copies can self-organize into large periodic or aperiodic systems. DNA is a particularly powerful system for this type of organization, because it is possible to flank structural motifs with sticky ends, so that Watson-Crick complementary interactions can be programmed to establish the intermolecular contacts. 1 DNA double crossover (DX) motifs are examples of small tiles (~ 4 nm×~ 16 nm) that have been programmed to produce 2D crystals; 4 often these tiles contain pattern-forming features when more than a single tile constitutes the crystallographic repeat. These motifs contain two parallel double helices, held together by crossovers; the second dimension derives from connecting one helix of a given tile to the other helix of an adjacent tile, as shown in Figure 1a. In addition to the periodic pattern shown there, this form of intermolecular organization has been used to
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38.3
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