Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists.
Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists.
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DOI：
10.1038/s41598-017-12480-7
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发表时间：
2017-10-09
影响因子：
4.6
通讯作者：
Rausch-Fan X
中科院分区：
文献类型：
--
作者：
Andrukhov O;Hong JS;Andrukhova O;Blufstein A;Moritz A;Rausch-Fan X
牙周膜干细胞与其他组织的间充质干细胞相似，具有免疫调节特性，受不同细胞因子的调节，尤其是受干扰素-γ(干扰素-γ)的调节。相反，关于Toll样受体配体对这些细胞免疫调节特性的影响提供的信息较少。在本研究中，我们研究了人牙周膜干细胞对干扰素-γ和Toll样受体激动剂同时刺激的反应。检测吲哚胺-2，3-双加氧酶-1(IDO-1)、白介素6(IL-6)、白介素8(IL-8)和单核细胞趋化蛋白-1(MCP-1)的表达。干扰素-γ可在基因和蛋白水平上调Ido-1的表达。TLR2激动剂Pam3CSK4诱导IDO-1基因表达，但对蛋白表达无影响。Pam3CSK4可进一步增强干扰素-γ诱导的IDO-1蛋白表达。TLR4激动剂E.ColiLPS对基础或干扰素-γ诱导的Ido-1蛋白表达均无明显影响。TLR激动剂诱导IL-6、IL-8和MCP-1的产生。基础激动剂和受体激动剂诱导的这些蛋白的产生均不受干扰素-γ的影响。我们的数据显示在hPDLSCs中干扰素-γ和TLR2反应之间存在潜在的相互作用，这可能参与炎症性疾病，特别是牙周炎的免疫反应的调节。
Periodontal ligament stem cells similarly to the mesenchymal stem cells of other tissues possess immunomodulatory properties, which are regulated by different cytokines, particularly by interferon-γ (IFN-γ). In contrast, less information is provided about the effect of toll-like receptors ligand on immunomodulatory properties of these cells. In the present study we investigated the response of human periodontal ligament stem cells (hPDLSCs) in response to simultaneous stimulation with IFN-γ and toll-like receptor (TLR) agonists. The resulting expression of indoleamine-2,3-dioxygenase-1 (IDO-1), interleukin (IL)-6, IL-8 and monocyte chemotactic protein 1 (MCP-1) was investigated. The expression of IDO-1 was upregulated by IFN-γ in both gene and protein levels. TLR2 agonist Pam3CSK4 induced gene expression of IDO-1, but had no effect on protein expression. IFN-γ induced IDO-1 protein expression was further enhanced by Pam3CSK4. TLR-4 agonist E. coli LPS has no significant effect on neither basal nor IFN-γ induced IDO-1 protein expression. The production of IL-6, IL-8, and MCP-1 was induced by TLR agonists. Neither basal nor TLR agonists induced production of these proteins was affected by IFN-γ. Our data shows potential interaction between IFN-γ and TLR2 responses in hPDLSCs, which might be involved in regulation of immune response in inflammatory diseases, and particularly periodontitis.
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影响因子：
3.7
作者：
Andrukhov O;Andrukhova O;Özdemir B;Haririan H;Müller-Kern M;Moritz A;Rausch-Fan X
通讯作者：
Rausch-Fan X
影响因子：
18.6
作者：
Kinane, DF
通讯作者：
Kinane, DF
影响因子：
7.3
作者：
Kawasaki T;Kawai T
通讯作者：
Kawai T
影响因子：
7.6
作者：
Huang GT;Gronthos S;Shi S
通讯作者：
Shi S
影响因子：
7.3
作者：
Delarosa O;Dalemans W;Lombardo E
通讯作者：
Lombardo E