Genes responding to water deficit in apple (Malus × domestica Borkh.) roots.
Genes responding to water deficit in apple (Malus × domestica Borkh.) roots.
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DOI：
10.1186/1471-2229-14-182
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发表时间：
2014-07-08
影响因子：
5.3
通讯作者：
Farrell RE Jr
中科院分区：
文献类型：
--
作者：
Bassett CL;Baldo AM;Moore JT;Jenkins RM;Soffe DS;Wisniewski ME;Norelli JL;Farrell RE Jr
个别植物利用生化、形态和生命周期策略的组合来适应其直接环境。由于木本植物是多年生植物，它们不能仅依靠年度生命周期策略来生存非生物胁迫。在这项研究中，我们使用抑制消减杂交技术来鉴定在缺水处理和恢复过程中在根中上调和下调的基因。此外，我们还跟踪了‘Royal Gala’苹果在模拟干旱和随后的恢复过程中根、叶、树皮和木质部中选择基因的表达。与草本植物和木本植物的研究一致，识别出了一些常见的干旱反应基因，以及一些以前没有报道过的基因。选择了三个基因进行更深入的分析：高亲和力硝酸盐转运蛋白(MdNRT2.4)、线粒体外膜转位酶(MdTOM7.1)和编码NPR1同源物的基因(MpNPR1-2)。这些基因在苹果根、树皮和叶片中的数量表达与它们在营养和防御中的作用是一致的。与之前来自同一器官的EST分析相比，利用抑制消减杂交技术鉴定了苹果根系对干旱做出反应的其他基因。还鉴定了在干旱恢复过程中根中上调和下调的基因。在根中发现了高亲和力硝酸盐转运体的水平升高，这表明由于干旱处理根中硝酸盐浓度的预测降低，氮素吸收从低亲和力转运体转移到了低亲和力转运体。干旱处理的苹果树叶片中NPR1基因的抑制可能在一定程度上解释了脱水条件下树木对疾病的敏感性增加。
Individual plants adapt to their immediate environment using a combination of biochemical, morphological and life cycle strategies. Because woody plants are long-lived perennials, they cannot rely on annual life cycle strategies alone to survive abiotic stresses. In this study we used suppression subtractive hybridization to identify genes both up- and down-regulated in roots during water deficit treatment and recovery. In addition we followed the expression of select genes in the roots, leaves, bark and xylem of ‘Royal Gala’ apple subjected to a simulated drought and subsequent recovery. In agreement with studies from both herbaceous and woody plants, a number of common drought-responsive genes were identified, as well as a few not previously reported. Three genes were selected for more in depth analysis: a high affinity nitrate transporter (MdNRT2.4), a mitochondrial outer membrane translocase (MdTOM7.1), and a gene encoding an NPR1 homolog (MpNPR1-2). Quantitative expression of these genes in apple roots, bark and leaves was consistent with their roles in nutrition and defense. Additional genes from apple roots responding to drought were identified using suppression subtraction hybridization compared to a previous EST analysis from the same organ. Genes up- and down-regulated during drought recovery in roots were also identified. Elevated levels of a high affinity nitrate transporter were found in roots suggesting that nitrogen uptake shifted from low affinity transport due to the predicted reduction in nitrate concentration in drought-treated roots. Suppression of a NPR1 gene in leaves of drought-treated apple trees may explain in part the increased disease susceptibility of trees subjected to dehydrative conditions.
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影响因子：
5.1
作者：
Dubos, C;Plomion, C
通讯作者：
Plomion, C
影响因子：
7.4
作者：
Bogeat-Triboulot, Marie-Beatrice;Brosche, Mikael;Dreyer, Erwin
通讯作者：
Dreyer, Erwin
影响因子：
4.9
作者：
Hattori, T;Totsuka, M;Yamamoto-Toyoda, A
通讯作者：
Yamamoto-Toyoda, A
DOI：
10.1073/pnas.95.11.6531
发表时间：
1998-05-26
影响因子：
11.1
作者：
Cao, H;Li, X;Dong, XN
通讯作者：
Dong, XN
影响因子：
5.1
作者：
Beuning, LL;Bowen, JH;MacRae, EA
通讯作者：
MacRae, EA