Improved Tapaswini having four BB resistance genes pyramided with six genes/QTLs, resistance/tolerance to biotic and abiotic stresses in rice.
Improved Tapaswini having four BB resistance genes pyramided with six genes/QTLs, resistance/tolerance to biotic and abiotic stresses in rice.
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DOI：
10.1038/s41598-018-20495-x
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发表时间：
2018-02-05
影响因子：
4.6
通讯作者：
Prasad D
中科院分区：
文献类型：
--
作者：
Das G;Rao GJN;Varier M;Prakash A;Prasad D
水稻是一种主要的粮食作物，在各种生态条件下种植，由于不断受到各种环境和生物胁迫，其产量损失很大。不同生物型/菌株的普遍存在，需要将许多抗性基因/ qtl组装成精英基因型，以赋予更广泛的抗性。目前的研究报告成功地构建了具有耐淹（Sub1）、耐盐（Saltol）、抗稻瘟病（Pi2、Pi9）和瘿蚊（Gm1、Gm4）抗性的基因/ qtl，以补充优良品种改良Tapaswini中存在的4种抗白叶枯病基因（Xa 4、xa5、xa13、Xa21）。通过有效的前景选择实现了基因/ qtl的精确转移，并确定了合适的基因金字塔。利用形态性状和籽粒品质性状进行背景选择，提高亲本基因组的复现率。在生物测定中，金字塔对目标应力表现出更高水平的抗性/耐受性。该研究的新特点是在一个新的基因型中成功的金字塔化和展示了10个基因/ qtl的功能。这一成功可以激发更多类似的研究，以充分发挥分子植物育种作为水稻改良基础的潜力。
Rice, a major food crop, is grown in a wide range of ecological conditions and suffers significant yield losses as it is constantly exposed to a wide range of environmental and biotic stresses. The prevalence of different biotypes/strains has necessitated assembling of numerous resistance genes/QTLs into elite genotypes to confer a broader scale of resistance. The current study reports successful pyramiding of genes/QTLs that confer tolerance/resistance to submergence (Sub1), salinity (Saltol), blast (Pi2, Pi9) and gall midge (Gm1, Gm4) to supplement the four bacterial blight resistance genes (Xa 4, xa5, xa13, Xa21) present in Improved Tapaswini, an elite cultivar. The precise transfer of genes/QTLs was accomplished through effective foreground selection and suitable gene pyramids were identified. Background selection was practiced using morphological and grain quality traits to enhance the recovery of the recurrent parental genome. In the bioassays, the pyramids exhibited higher levels of resistance/ tolerance against the target stresses. The novel feature of the study was successful pyramidization and demonstration of the function of ten genes/QTLs in a new genotype. This success can stimulate several such studies to realize the full potential of molecular plant breeding as the foundation for rice improvement.
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影响因子：
5.6
作者：
Das G;Rao GJ
通讯作者：
Rao GJ
影响因子：
3.7
作者：
Campbell MT;Proctor CA;Dou Y;Schmitz AJ;Phansak P;Kruger GR;Zhang C;Walia H
通讯作者：
Walia H
影响因子：
5.8
作者：
Ali, AJ;Xu, JL;Li, ZK
通讯作者：
Li, ZK
影响因子：
3.1
作者：
ANDERSON, JA;CHURCHILL, GA;SORRELLS, ME
通讯作者：
SORRELLS, ME
影响因子：
4.9
作者：
FAGERIA, NK
通讯作者：
FAGERIA, NK