Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS
Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS
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小麦染色体臂 2BS 上推测的中间偃麦草来源的显性白粉病抗性基因 PmL962 的鉴定和遗传图谱
DOI：
10.1007/s00122-014-2449-x
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发表时间：
2015-01
影响因子：
5.4
通讯作者：
Luo PG
中科院分区：
文献类型：
--
作者：
Zhang HY;Tan FQ;Ren ZL;Luo PG
小麦品系L962的抗粉性来源于中间偃麦草，由一对显性基因PmL 962控制，定位于染色体2BS臂。小麦白粉病是由Blumeria graminis f.小麦赤霉病（Triticum aestivum sp. tritici，Bgt）是一种严重危害小麦生产的病害。结果表明，中间偃麦草（Thinopyrum intermedium）对白粉病的抗性已转移到普通小麦品系L962上，该品系对多个中国Bgt菌株具有抗性。通过将抗性品系L962与感病品系L983杂交进行白粉病反应的遗传分析。对L983/L962杂交组合的F1、F2和F2：3群体的病害评估表明，抗性由单个显性基因控制。利用373个F2：3品系和781对SSR引物对该抗性基因进行了染色体定位。该基因与4个公开和新近开发的小麦基因组SSR标记和7个EST-STS标记连锁。根据连锁标记的位置将抗性基因定位于染色体臂2BS。系谱、分子标记和抗性反应数据表明，L962中的抗白粉病基因是一个新基因。它被暂时命名为PmL 962。它的侧翼是Xwmc 314和BE 443737，遗传距离分别为2.09和3.74cM，并位于20.77cM的区间内，与二穗短柄草第5染色体上的269.4kb基因组区域和水稻第4染色体上的223.5kb基因组区域共线性。与该基因紧密连锁的分子标记在分子标记辅助育种中具有潜在的应用价值。
Powdery resistance putatively derived from Thinopyrum intermedium in the wheat line L962 is controlled by a single dominant gene designated PmL962 and mapped to chromosome arm 2BS. Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease affecting the production of wheat (Triticum aestivum). Powdery mildew resistance was putatively transferred from Thinopyrum intermedium to the common wheat line L962, which conferred resistance to multiple Chinese Bgt isolates. Genetic analysis of the powdery mildew response was conducted by crossing the resistant line L962 with the susceptible line L983. Disease assessments of the F1, F2, and F2:3 populations from the cross L983/L962 indicated that resistance was controlled by a single dominant gene. A total of 373 F2:3 lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to four publicly available and recently developed wheat genomic SSR markers and seven EST-STS markers. The resistance gene was mapped to chromosome arm 2BS based on the locations of the linked markers. Pedigree, molecular marker and resistance response data indicated that the powdery mildew resistance gene in L962 is novel. It was temporarily designated PmL962. It is flanked by Xwmc314 and BE443737at genetic distances of 2.09 and 3.74 cM, respectively, and located in a 20.77 cM interval that is co-linear with a 269.4 kb genomic region on chromosome 5 in Brachypodium distachyon and a 223.5 kb genomic region on rice (Oryza sativa) chromosome 4. The markers that are closely linked to this gene have potential applications in marker-assisted breeding.
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影响因子：
3.1
作者：
Fedak, G
通讯作者：
Fedak, G
影响因子：
64.8
作者：
Vogel, John P.;Garvin, David F.;Baxter, Ivan
通讯作者：
Baxter, Ivan
影响因子：
2.3
作者：
Kuraparthy, Vasu;Sood, Shilpa;Gill, Bikram S.
通讯作者：
Gill, Bikram S.
DOI：
10.1073/pnas.1016981108
发表时间：
2011-05-10
影响因子：
11.1
作者：
Cao, Aizhong;Xing, Liping;Chen, Peidu
通讯作者：
Chen, Peidu
影响因子：
0.8
作者：
Liu, Zhaohui;Xu, Meng;Luo, Peigao
通讯作者：
Luo, Peigao