Comparison of lower genital tract microbiota in HIV-infected and uninfected women from Rwanda and the US.
Comparison of lower genital tract microbiota in HIV-infected and uninfected women from Rwanda and the US.
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DOI：
10.1371/journal.pone.0096844
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Spear GT
中科院分区：
文献类型：
--
作者：
Benning L;Golub ET;Anastos K;French AL;Cohen M;Gilbert D;Gillevet P;Munyazesa E;Landay AL;Sikaroodi M;Spear GT
先前的研究表明，女性下生殖道细菌微生物群的改变会影响HIV感染和脱落的易感性。我们评估了来自卢旺达和美国的hiv感染妇女和未感染妇女生殖器微生物群类型的地理差异。通过对46名美国妇女（36名hiv感染者，10名hiv未感染者）和40名卢旺达妇女（18名hiv感染者，22名hiv未感染者）的16S rRNA基因进行高通量焦磷酸测序，鉴定了下生殖道细菌微生物群属，其纽金特评分低（0-3）的比例相似。将序列与参考序列组装成系统发育树，鉴定乳酸菌的种类。采用Fisher精确检验法比较了乳酸菌属和菌种的流行情况。总的来说，七个最常见的属是乳酸菌（74%），普雷沃菌（56%），加德纳菌（55%），阿托波菌（42%），Sneathia (37%), Megasphaera（30%）和Parvimonas(26%)，除了Megasphaera(20%对39%，p = 0.06)，在卢旺达和美国女性中观察到相似的患病率。此外，与美国妇女相比，卢旺达妇女的支原体（23%比7%，p = 0.06）和蛋菌（13%比0%，p = 0.02）的发病率更高，而毛杆菌（8%比35%，p<0.01）和Allisonella（5%比30%，p<0.01）的发病率较低。支原体感染率在感染艾滋病毒的卢旺达妇女（39%）中最高（p<0.05），而感染艾滋病毒的美国妇女（6%）、未感染艾滋病毒的卢旺达妇女（9%）和美国妇女（10%）。卢旺达和美国妇女中最常见的乳酸菌种是乳杆菌（58%对76%，p = 0.11），其次是crispatus乳杆菌（28%对30%，p = 0.82）， jensenii乳杆菌（20%对24%，p = 0.80）， L. gasseri乳杆菌（20%对11%，p = 0.37）和阴道乳杆菌（20%对7%，p = 0.10）。我们发现卢旺达和美国妇女中大多数主要细菌属和乳杆菌种类的患病率相似。需要进一步的工作来确定观察到的差异是否会对下生殖道健康或对生殖道感染的易感性产生不同的影响。
Previous studies have shown that alterations of the bacterial microbiota in the lower female genital tract influence susceptibility to HIV infection and shedding. We assessed geographic differences in types of genital microbiota between HIV-infected and uninfected women from Rwanda and the United States. Genera of lower genital tract bacterial microbiota were identified by high-throughput pyrosequencing of the 16S rRNA gene from 46 US women (36 HIV-infected, 10 HIV-uninfected) and 40 Rwandan women (18 HIV-infected, 22 HIV-uninfected) with similar proportions of low (0–3) Nugent scores. Species of Lactobacillus were identified by assembling sequences along with reference sequences into phylogenetic trees. Prevalence of genera and Lactobacillus species were compared using Fisher's exact tests. Overall the seven most prevalent genera were Lactobacillus (74%), Prevotella (56%), Gardnerella (55%), Atopobium (42%), Sneathia (37%), Megasphaera (30%), and Parvimonas (26%), observed at similar prevalences comparing Rwandan to US women, except for Megasphaera (20% vs. 39%, p = 0.06). Additionally, Rwandan women had higher frequencies of Mycoplasma (23% vs. 7%, p = 0.06) and Eggerthella (13% vs. 0%, p = 0.02), and lower frequencies of Lachnobacterium (8% vs. 35%, p<0.01) and Allisonella (5% vs. 30%, p<0.01), compared with US women. The prevalence of Mycoplasma was highest (p<0.05) in HIV-infected Rwandan women (39%), compared to HIV-infected US women (6%), HIV-uninfected Rwandan (9%) and US (10%) women. The most prevalent lactobacillus species in both Rwandan and US women was L. iners (58% vs. 76%, p = 0.11), followed by L. crispatus (28% vs. 30%, p = 0.82), L. jensenii (20% vs. 24%, p = 0.80), L. gasseri (20% vs. 11%, p = 0.37) and L. vaginalis (20% vs. 7%, p = 0.10). We found similar prevalence of most major bacterial genera and Lactobacillus species in Rwandan and US women. Further work will be needed to establish whether observed differences differentially impact lower genital tract health or susceptibility to genital infections.
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影响因子：
17.1
作者：
Gajer P;Brotman RM;Bai G;Sakamoto J;Schütte UM;Zhong X;Koenig SS;Fu L;Ma ZS;Zhou X;Abdo Z;Forney LJ;Ravel J
通讯作者：
Ravel J
影响因子：
5.4
作者：
Barkan, SE;Melnick, SL;Feldman, J
通讯作者：
Feldman, J
影响因子：
3.7
作者：
Jakobsen TH;Hansen MA;Jensen PØ;Hansen L;Riber L;Cockburn A;Kolpen M;Rønne Hansen C;Ridderberg W;Eickhardt S;Hansen M;Kerpedjiev P;Alhede M;Qvortrup K;Burmølle M;Moser C;Kühl M;Ciofu O;Givskov M;Sørensen SJ;Høiby N;Bjarnsholt T
通讯作者：
Bjarnsholt T
影响因子：
4.4
作者：
Ling Z;Kong J;Liu F;Zhu H;Chen X;Wang Y;Li L;Nelson KE;Xia Y;Xiang C
通讯作者：
Xiang C
DOI：
10.1073/pnas.1000086107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Macklaim, Jean M.;Gloor, Gregory B.;Reid, Gregor
通讯作者：
Reid, Gregor