An integrated microfluidic chip for treatment and detection of microalgae cells
An integrated microfluidic chip for treatment and detection of microalgae cells
复制标题
用于微藻细胞处理和检测的集成微流控芯片
DOI：
10.1016/j.algal.2019.101593
复制
发表时间：
2019-09
影响因子：
5.1
通讯作者：
Pan Xinxiang
中科院分区：
文献类型：
--
作者：
Wang Junsheng;Wang Ge;Chen Mengmeng;Wang Yanjuan;Ding Gege;Zhang Yichi;Kang Yuejun;Pan Xinxiang
海运作为大宗货物最经济的国际运输方式，对世界贸易和经济做出了巨大贡献。然而压载水中携带大量微藻细胞和其他外来生物，可能严重破坏船舶运输航线沿线的海洋生态环境。微藻细胞和富含微藻的压载水的在线处理已成为压载水管理的迫切需求。在此，我们开发了一种基于激光诱导叶绿素荧光的自动微流体系统，用于实时检测压载水样品中的微藻。与需要繁琐的操作程序、延长的检测时间和复杂的分析过程的传统方法相比，该系统具有很大的优势。此外，我们引入了化学方法来快速有效地处理压载水中的微藻细胞。为此，使用微流体浓度梯度发生器快速筛选化学物质的最佳浓度，以快速减少相应压载水中的微藻种群。据我们所知，这是首次将自动高通量检测系统与梯度浓度芯片相结合用于压载水的原位分析和处理。
As the most economic means of international transportation for massive cargo, marine transportation contributes significantly to the world trading and economics. However, there are large number of microalgae cells and other exotic organisms carried in the ballast water, which may seriously damage the marine ecological environment along the transportation routes of the ships. Microalgae cells and online treatment of the microalgae-rich ballast water have become a pressing demand for ballast water management. Herein, we developed an automatic microfluidic system based on laser-induced chlorophyll fluorescence for real-time detection of microalgae in ballast water samples. This system is highly advantageous compared to the conventional method that requires tedious operation procedures, prolonged detection time, and complex analytical process. Moreover, we introduced chemical methods for fast and effective treatment of the microalgae cells in ballast water. For this purpose, a microfluidic concentration gradient generator was used for fast screening the optimal concentration of chemicals to rapidly reduce the microalgae population in the corresponding ballast water. To the best of our knowledge, this is the first time to combine an automatic high-throughput detection system and a gradient concentration chip for in situ analysis and treatment of ballast water.
登录
查看更多内容
DOI：
10.1002/j.1551-8833.2011.tb11474.x
发表时间：
2011-06
期刊：
Journal ‐ American Water Works Association
影响因子：
--
作者：
Benjamin D. Stanford;A. Pisarenko;S. Snyder;G. Gordon
通讯作者：
Benjamin D. Stanford;A. Pisarenko;S. Snyder;G. Gordon
影响因子：
5.8
作者：
David, M;Perkovic, M
通讯作者：
Perkovic, M
在动态环境中长期监测藻类的微流体平台。
影响因子：
4.7
作者：
Luke CS;Selimkhanov J;Baumgart L;Cohen SE;Golden SS;Cookson NA;Hasty J
通讯作者：
Hasty J
DOI：
10.1016/j.snb.2018.03.091
发表时间：
2018-08
期刊：
Sensors and Actuators B: Chemical
影响因子：
--
作者：
Ruey-Jen Yang;Lung-Ming Fu;H. Hou
通讯作者：
Ruey-Jen Yang;Lung-Ming Fu;H. Hou
DOI：
10.3390/s151229848
发表时间：
2015-12-10
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Perestrelo AR;Águas AC;Rainer A;Forte G
通讯作者：
Forte G