Tapetal-Delayed Programmed Cell Death (PCD) and Oxidative Stress-Induced Male Sterility of Aegilops uniaristata Cytoplasm in Wheat.
Tapetal-Delayed Programmed Cell Death (PCD) and Oxidative Stress-Induced Male Sterility of Aegilops uniaristata Cytoplasm in Wheat.
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小麦绒毡层延迟程序性细胞死亡（PCD）和氧化应激诱导的山羊草细胞质雄性不育
DOI：
10.3390/ijms19061708
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发表时间：
2018-06-08
影响因子：
5.6
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Liu Z;Shi X;Li S;Hu G;Zhang L;Song X
细胞质雄性不育在杂种优势利用中起着至关重要的作用。花粉发育过程中常伴随着氧化代谢反应和绒毡层细胞程序性死亡（PCD），这些过程的缺失可导致雄性不育。单芒山羊草细胞质雄性不育小麦是一种新型的小麦雄性不育系，在杂交小麦育种中具有重要的应用潜力。然而，其CMS机制仍然知之甚少。本研究以单芒山羊草细胞质U87 B1 - 706 A和保持系706 B为材料，探讨了其败育原因。与706 B相比，U87 B1 - 706 A花药的组织学分析和PCD检测结果表明，U87 B1 - 706 A在单核期早期表现为绒毡层PCD延迟和细胞器结构紊乱的表型。单核晚期，小孢子皱缩，外壁结构紊乱。U87 B1 - 706 A花粉发育过程中，活性氧（ROS）的过度积累导致抗氧化酶活性显著升高，非酶抗氧化物质含量显著下降。实时荧光定量PCR检测结果表明，在花粉发育早期，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）基因的转录水平上调。因此，我们推测过量的ROS可能与酶基因表达水平的提高有关，从而打破了抗氧化系统的平衡，导致绒毡层PCD启动延迟，最终导致花粉败育和雄性不育。这些结果为进一步探讨不育小麦花粉败育的机理提供了依据。
Cytoplasmic male sterility (CMS) plays a crucial role in the utilization of hybrid vigor. Pollen development is often accompanied by oxidative metabolism responses and tapetal programmed cell death (PCD), and deficiency in these processes could lead to male sterility. Aegilops uniaristata cytoplasmic male sterility (Mu-CMS) wheat is a novel male-sterile line in wheat, which possess important potential in hybrid wheat breeding. However, its CMS mechanisms remain poorly understood. In our study, U87B1-706A, with the Aegilops uniaristata cytoplasm, and the maintainer line 706B were used to explore the abortive reason. Compared with 706B, histological analysis and PCD detection of the anther demonstrated that U87B1-706A appeared as delayed tapetal PCD as well as a disorganized organelle phenotype in the early uninucleate stage. Subsequently, a shrunken microspore and disordered exine structure were exhibited in the late uninucleate stage. While the activities of antioxidase increased markedly, the nonenzymatic antioxidant contents declined obviously following overacummulation of reactive oxygen species (ROS) during pollen development in U87B1-706A. Real-time quantitative PCR testified that the transcript levels of the superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) genes, encoding pivotal antioxidant enzymes, were up-regulated in early pollen development. Therefore, we deduce excess ROS as a signal may be related to the increased expression levels of enzyme genes, thereby breaking the antioxidative system balance, resulting in delayed tapetal PCD initiation, which finally led to pollen abortion and male sterility in U87B1-706A. These results provide evidence to further explore the mechanisms of abortive pollen in CMS wheat.
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影响因子：
4.2
作者：
Falasca G;D'Angeli S;Biasi R;Fattorini L;Matteucci M;Canini A;Altamura MM
通讯作者：
Altamura MM
影响因子：
5.6
作者：
Ji JJ;Huang W;Li Z;Chai WG;Yin YX;Li DW;Gong ZH
通讯作者：
Gong ZH
影响因子：
3
作者：
Li, Jianxin;Dai, Ximei;Huang, Qunce
通讯作者：
Huang, Qunce
影响因子：
4.2
作者：
Bhoomika, Kumari;Pyngrope, Samantha;Dubey, R. S.
通讯作者：
Dubey, R. S.
影响因子：
2.6
作者：
Panda, Sanjib Kumar;Sahoo, Lingaraj;Matsumoto, Hideaki
通讯作者：
Matsumoto, Hideaki