Type X collagen synthesis by chick sternal cartilage and its relationship to endochondral development.
Type X collagen synthesis by chick sternal cartilage and its relationship to endochondral development.
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X型胶原蛋白胸骨软骨的合成及其与内骨的关系的关系。
DOI：
10.1083/jcb.101.1.277
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发表时间：
1985-07
影响因子：
7.8
通讯作者：
FLINT, MH
中科院分区：
文献类型：
--
作者：
GIBSON, GJ;FLINT, MH
我们的形态学研究表明，出现本地化，配对区的初级钙化的中线两侧的19-D的鸡胚胸骨是预示着发展的配对，半透明区2天以前。组织学研究表明，这些半透明区中的大多数软骨细胞是肥大的，并且这些区被扁平的非肥大细胞边缘包围。这些成对的肥大软骨细胞区域和随后的软骨内骨发育的离散定位允许在发育期间顺序地将这些区域的组织学和生化特征直接关联，并且使得有可能精确地将合成活性与细胞形态相匹配，从而消除否则可能出现的发育分期中可能的微小但关键的变化。我们的研究表明，细胞成熟程度与X型胶原蛋白的合成之间存在直接的空间和时间相关性，并且在发育第16-17天X型胶原蛋白合成的突然而深刻的启动与获得肥大同时发生。半透明区内的大多数细胞具有肥大特征。在获得这些肥大特征之前，该预钙化区的细胞仅合成II型和少量软骨胶原。从更不成熟的胸骨中的这些区域分离的软骨细胞（即，11+ d胚胎）在胶原凝胶内培养4 d内合成高水平的X型胶原，即使该区域内细胞的肥大发育和X型胶原合成在卵内正常情况下在几天内不会明显。这些数据表明，有一个直接的相关性之间的发展，肥大的特点和X型胶原的合成，并在钙化区的软骨细胞的成熟，可以调节基质成分和/或刺激内胶原凝胶培养。
Our morphological studies have demonstrated that the appearance of localized, paired zones of primary calcification on either side of the midline of the 19-d embryonic chick sternum is heralded by the development of paired, translucent zones 2 d previously. Histological studies demonstrated that the majority of chondrocytes within these translucent zones are hypertrophic, and that the zones are surrounded by a margin of flattened nonhypertrophic cells. The discrete localization of these paired areas of hypertrophic chondrocytes and subsequent endochondral bone development allows for the direct correlation of the histological and biochemical characteristics of the zones sequentially during development and makes it possible to precisely match the synthetic activity to the cellular morphology, thereby eliminating possible minor but critical variations in developmental staging that could otherwise arise. Our studies have demonstrated that there is a direct spatial and temporal correlation between the degree of cellular maturation and the synthesis of type X collagen, and that the sudden and profound initiation of type X collagen synthesis on days 16-17 of development occurs concurrently with the attainment of hypertrophic characteristics by the majority of cells within the translucent zone. Before acquisition of these hypertrophic characteristics, the cells of this precalcification zone synthesize only type II and the minor cartilage collagens. Chondrocytes isolated from these regions in more immature sternae (i.e., 11+ d embryos) were found to synthesize high levels of type X collagen within 4 d of culture within collagen gels even though hypertrophic development and type X collagen synthesis by cells within this region would not normally have been apparent in ovo for several more days. These data indicate that there is a direct correlation between the development of hypertrophic characteristics and the synthesis of type X collagen, and that the maturation of chondrocytes in precalcification zones may be regulated by matrix components and/or stimulated by culture within collagen gels.
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基质大分子对软骨细胞基因表达的影响：通过在胶原蛋白内培养的细胞合成低分子量胶原蛋白物种。
影响因子：
7.8
作者：
Gibson, G J;Schor, S L;Grant, M E
通讯作者：
Grant, M E
影响因子：
3.5
作者：
SHIMOKOMAKI, M;DUANCE, VC;BAILEY, AJ
通讯作者：
BAILEY, AJ
影响因子：
3.7
作者：
CAPASSO, O;GIONTI, E;CANCEDDA, R
通讯作者：
CANCEDDA, R
影响因子：
3.5
作者：
KIELTY, CM;HULMES, DJS;GRANT, ME
通讯作者：
GRANT, ME
影响因子：
2.9
作者：
REESE, CA;WIEDEMANN, H;MAYNE, R
通讯作者：
MAYNE, R