MEMS-based handheld fourier domain Doppler optical coherence tomography for intraoperative microvascular anastomosis imaging.
MEMS-based handheld fourier domain Doppler optical coherence tomography for intraoperative microvascular anastomosis imaging.
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DOI：
10.1371/journal.pone.0114215
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kang JU
中科院分区：
文献类型：
--
作者：
Huang Y;Furtmüller GJ;Tong D;Zhu S;Lee WP;Brandacher G;Kang JU
目的：探讨微型手持式光学相干断层扫描（OCT）成像仪在超显微外科血管吻合中实时评估术中血管通畅程度的可行性。开发了一种基于1.3µm中心波长扫频源的新型手持式傅里叶域多普勒光学相干断层成像技术。通过采用直径2.4 mm的微机电系统（MEMS）扫描镜，将成像仪最小化，另外设计了直径12.7 mm的透镜系统，并与MEMS扫描镜相结合，实现了小尺寸的外形，优化了手持式血管外OCT成像仪的功能。为了评估在体内的适用性，我们采用传统的间断缝合技术和一种新型的非缝合袖带技术对小鼠股血管切割和修复模型进行了超显微外科血管吻合。应用新型手持式OCT成像仪对临床成功修复后血管吻合口通畅程度进行评价。利用1.5 mm × 1.5 mm的可调节侧位图像视场，成功获得了BALB/C小鼠原位后肢移植后采用非缝合袖带技术和BALB/C小鼠股动脉吻合后采用缝合技术的高分辨率血管结构和血流成像。我们通过实验将OCT的轴向和横向分辨率分别量化为空气中的12.6µm和17.5µm。OCT的灵敏度为84 dB，在5 mm的成像范围内灵敏度滚降为5.7 dB/mm。成像帧率为36 Hz，图像尺寸为1000（横向）×512（轴向）像素，使用每秒50,000条a线扫描源。根据获得的血管结构和多普勒图像，定量分析血管管腔通畅、管腔狭窄和血栓形成。成功地展示了一种可用于术中微血管吻合评价的微型手持式OCT成像仪。
To demonstrate the feasibility of a miniature handheld optical coherence tomography (OCT) imager for real time intraoperative vascular patency evaluation in the setting of super-microsurgical vessel anastomosis. A novel handheld imager Fourier domain Doppler optical coherence tomography based on a 1.3-µm central wavelength swept source for extravascular imaging was developed. The imager was minimized through the adoption of a 2.4-mm diameter microelectromechanical systems (MEMS) scanning mirror, additionally a 12.7-mm diameter lens system was designed and combined with the MEMS mirror to achieve a small form factor that optimize functionality as a handheld extravascular OCT imager. To evaluate in-vivo applicability, super-microsurgical vessel anastomosis was performed in a mouse femoral vessel cut and repair model employing conventional interrupted suture technique as well as a novel non-suture cuff technique. Vascular anastomosis patency after clinically successful repair was evaluated using the novel handheld OCT imager. With an adjustable lateral image field of view up to 1.5 mm by 1.5 mm, high-resolution simultaneous structural and flow imaging of the blood vessels were successfully acquired for BALB/C mouse after orthotopic hind limb transplantation using a non-suture cuff technique and BALB/C mouse after femoral artery anastomosis using a suture technique. We experimentally quantify the axial and lateral resolution of the OCT to be 12.6 µm in air and 17.5 µm respectively. The OCT has a sensitivity of 84 dB and sensitivity roll-off of 5.7 dB/mm over an imaging range of 5 mm. Imaging with a frame rate of 36 Hz for an image size of 1000(lateral)×512(axial) pixels using a 50,000 A-lines per second swept source was achieved. Quantitative vessel lumen patency, lumen narrowing and thrombosis analysis were performed based on acquired structure and Doppler images. A miniature handheld OCT imager that can be used for intraoperative evaluation of microvascular anastomosis was successfully demonstrated.
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