Carbon nanotubes-based chemiresistive immunosensor for small molecules: detection of nitroaromatic explosives.
Carbon nanotubes-based chemiresistive immunosensor for small molecules: detection of nitroaromatic explosives.
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DOI：
10.1016/j.bios.2010.07.017
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发表时间：
2010-12-15
影响因子：
12.6
通讯作者：
Mulchandani A
中科院分区：
文献类型：
--
作者：
Park M;Cella LN;Chen W;Myung NV;Mulchandani A
近年来，人们越来越关注使用一维（1-D）纳米结构，如碳纳米管和纳米线，作为无标记化学电阻/场效应晶体管生物传感器的换能器元件，因为它们提供无标记和高灵敏度检测。虽然迄今为止的研究已经阐明了碳纳米管和其他基于1-D纳米结构的场效应晶体管免疫传感器用于大的带电大分子如蛋白质和病毒的能力，但它们对小的不带电或带电分子的应用尚未得到证实。本文报道了一种基于单壁碳纳米管（SWNTs）的化学电阻免疫传感器，用于小分子2，4，6-三硝基甲苯（TNT）的无标记、快速、灵敏和选择性检测。新开发的免疫传感器采用位移模式/格式，其中形成传感器的传导通道的单壁碳纳米管网络首先用三硝基苯基（TNP）（TNT的类似物）修饰，然后与抗TNP单链抗体连接。在暴露于TNT或其衍生物时，结合的抗体被置换，在SWNT的电阻/电导中产生比噪声高几倍的大的变化，从而提供优异的检测限、灵敏度和选择性。该传感器检测到0.5 ppb和5000 ppb之间的TNT与其他硝基芳香族炸药具有良好的选择性，并表现出良好的准确性监测TNT在未经处理的环境水基质。我们相信这种新的位移格式可以很容易地推广到其他一维纳米结构为基础的化学电阻免疫/亲和传感器，用于检测小的和/或不带电的分子在环境监测和医疗保健的兴趣。
In recent years, there has been a growing focus on use of one-dimensional (1-D) nanostructures, such as carbon nanotubes and nanowires, as transducer elements for label-free chemiresistive/field-effect transistor biosensors as they provide label-free and high sensitivity detection. While research to-date has elucidated the power of carbon nanotubes- and other 1-D nanostructure- based field effect transistors immunosensors for large charged macromolecules such as proteins and viruses, their application to small uncharged or charged molecules has not been demonstrated. In this paper we report a single-walled carbon nanotubes (SWNTs)-based chemiresistive immunosensor for label-free, rapid, sensitive and selective detection of 2,4,6-trinitrotoluene (TNT), a small molecule. The newly developed immunosensor employed a displacement mode/format in which SWNTs network forming conduction channel of the sensor was first modified with trinitrophenyl (TNP), an analog of TNT, and then ligated with the anti-TNP single chain antibody. Upon exposure to TNT or its derivatives the bound antibodies were displaced producing a large change, several folds higher than the noise, in the resistance/conductance of SWNTs giving excellent limit of detection, sensitivity and selectivity. The sensor detected between 0.5 ppb and 5000 ppb TNT with good selectivity to other nitroaromatic explosives and demonstrated good accuracy for monitoring TNT in untreated environmental water matrix. We believe this new displacement format can be easily generalized to other one-dimensional nanostructure-based chemiresistive immuno/affinity-sensors for detecting small and/or uncharged molecules of interest in environmental monitoring and health care.
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影响因子：
--
作者：
Hangarter, Carlos M.;Bangar, Mangesh;Myung, Nosang V.
通讯作者：
Myung, Nosang V.
影响因子：
3
作者：
Lim, Jae-Hong;Phiboolsirichit, Nopparat;Myung, Nosang V.
通讯作者：
Myung, Nosang V.
影响因子：
8.4
作者：
Elkind, JL;Stimpson, DI;Melendez, JL
通讯作者：
Melendez, JL
影响因子：
8.4
作者：
Larsson, A;Angbrant, J;Liedberg, B
通讯作者：
Liedberg, B
影响因子：
4.1
作者：
Halasz, A;Groom, C;Hawari, J
通讯作者：
Hawari, J