Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.
Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.
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斑马鱼斑马鱼卵细胞质中的补体活性：母体补体成分防御作用的证据。
DOI：
10.1371/journal.pone.0001463
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发表时间：
2008-01-23
期刊：
影响因子：
3.7
通讯作者：
An Y
中科院分区：
文献类型：
--
作者：
Wang Z;Zhang S;Wang G;An Y
大多数在外部发育的鱼胚胎都暴露在充满微生物的环境中。它们是如何在微生物的攻击下存活至今还不清楚。本文通过建立的补体活性体外测定系统，证明了斑马鱼新受精卵制备的卵细胞溶胶能够杀死革兰氏阴性杆菌大肠杆菌。所有研究结果表明，这是补体系统通过替代途径运作，可归因于细菌的溶解活性。这是第一个为鱼卵中母体补体成分的功能作用提供证据的报告，为研究其他卵子体外受精生物的母体免疫铺平了道路。
Most fish embryos that develop externally are exposed to an environment full of microbes. How they survive microbial attacks are not understood to date. Here we demonstrated that the egg cytosol prepared from the newly fertilized eggs of zebrafish Danio rerio is capable of killing the Gram-negative bacterium Escherichia coli, via in vitro assay system of the complement activity established. All findings indicate that it is the complement system operating via the alternative pathway that is attributable to the bacteriolytic activity. This is the first report providing the evidence for the functional role of the maternal complement components in fish eggs, paving the way for study of maternal immunity in other organisms whose eggs are fertilized in vitro.
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通讯作者：
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