Comparative metabolomics analysis of different sesame (Sesamum indicum L.) tissues reveals a tissue-specific accumulation of metabolites.
Comparative metabolomics analysis of different sesame (Sesamum indicum L.) tissues reveals a tissue-specific accumulation of metabolites.
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DOI：
10.1186/s12870-021-03132-0
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发表时间：
2021-07-24
影响因子：
5.3
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Dossou SSK;Xu F;Cui X;Sheng C;Zhou R;You J;Tozo K;Wang L
芝麻（芝麻）叶，花，尤其是种子在传统医学中被用来预防或治疗各种疾病。它的种子市场正在扩大。然而，由于缺乏与植物中代谢物分布和变异性相关的信息，其他组织仍然未被充分利用。本文采用基于超高效液相色谱-质谱（UPLC-MS/MS）的广泛靶向代谢组学分析平台，研究了芝麻叶片、鲜籽、白色和紫色花、鲜心皮5种组织的代谢物谱。总共定性和定量鉴定了属于不同类别的776种代谢物。不同组织的代谢物组成存在明显差异。大部分黄酮类化合物主要积累在花中。氨基酸及其衍生物和脂质主要在新鲜种子中发现，其次是花。其代谢产物包括醌类、香豆素类、单宁类、维生素类、萜类和一些具有生物活性的酚酸类化合物（类叶升麻苷、异类叶升麻苷、毛蕊花苷、车前草苷等）。主要集中在叶子上。木脂素主要在种子中检测到。筛选出238个关键的显著差异代谢产物。KEGG注释和富集分析的差异代谢产物表明，黄酮类化合物的生物合成，氨基酸的生物合成，苯丙素类化合物的生物合成是主要的不同调节途径。除了代谢物的组织特异性积累，我们注意到叶，新鲜心皮和发育中的种子之间的代谢物转移的合作关系。飞燕草素-3-O-（6 ′-O-p-香豆酰基）葡萄糖苷和大部分黄酮醇在紫色花中表达上调，表明它们可能是紫色花着色的原因。这项研究表明，芝麻组织中的代谢过程受到不同的调节。它为研究芝麻组织中基因-代谢物的相互作用和研究芝麻种子发育过程中的代谢运输提供了宝贵的资源。此外，我们的研究结果提供了重要的知识，将促进芝麻生物质的价值。在线版本包含补充材料，可通过10.1186/s12870-021-03132-0获得。
Sesame (Sesamum indicum L.) leaves, flowers, especially seeds are used in traditional medicine to prevent or cure various diseases. Its seed’s market is expanding. However, the other tissues are still underexploited due to the lack of information related to metabolites distribution and variability in the plant. Herein, the metabolite profiles of five sesame tissues (leaves, fresh seeds, white and purple flowers, and fresh carpels) have been investigated using ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS)-based widely targeted metabolomics analysis platform. In total, 776 metabolites belonging to diverse classes were qualitatively and quantitatively identified. The different tissues exhibited obvious differences in metabolites composition. The majority of flavonoids predominantly accumulated in flowers. Amino acids and derivatives, and lipids were identified predominantly in fresh seeds followed by flowers. Many metabolites, including quinones, coumarins, tannins, vitamins, terpenoids and some bioactive phenolic acids (acteoside, isoacteoside, verbascoside, plantamajoside, etc.) accumulated mostly in leaves. Lignans were principally detected in seeds. 238 key significantly differential metabolites were filtered out. KEGG annotation and enrichment analyses of the differential metabolites revealed that flavonoid biosynthesis, amino acids biosynthesis, and phenylpropanoid biosynthesis were the main differently regulated pathways. In addition to the tissue-specific accumulation of metabolites, we noticed a cooperative relationship between leaves, fresh carpels, and developing seeds in terms of metabolites transfer. Delphinidin-3-O-(6ʺ-O-p-coumaroyl)glucoside and most of the flavonols were up-regulated in the purple flowers indicating they might be responsible for the purple coloration. This study revealed that the metabolic processes in the sesame tissues are differently regulated. It offers valuable resources for investigating gene-metabolites interactions in sesame tissues and examining metabolic transports during seed development in sesame. Furthermore, our findings provide crucial knowledge that will facilitate sesame biomass valorization. The online version contains supplementary material available at 10.1186/s12870-021-03132-0.
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影响因子：
9.2
作者：
Hussain G;Rasul A;Anwar H;Aziz N;Razzaq A;Wei W;Ali M;Li J;Li X
通讯作者：
Li X
影响因子：
5.4
作者：
Chukwuma, Chika Ifeanyi;Matsabisa, Motlalepula G.;Islam, Md Shahidul
通讯作者：
Islam, Md Shahidul
一种用于大规模检测、鉴定和定量广泛目标代谢物的新型综合方法：在水稻代谢组学研究中的应用。
影响因子：
27.5
作者：
Chen, Wei;Gong, Liang;Luo, Jie
通讯作者：
Luo, Jie
影响因子：
3.3
作者：
Jaiswal, Yogini;Liang, Zhitao;Zhao, Zhongzhen
通讯作者：
Zhao, Zhongzhen
影响因子：
5.2
作者：
Hata, Naoki;Hayashi, Yoshinori;Kobayashi, Akio
通讯作者：
Kobayashi, Akio