Colorimetric-Luminance Readout for Quantitative Analysis of Fluorescence Signals with a Smartphone CMOS Sensor.
Colorimetric-Luminance Readout for Quantitative Analysis of Fluorescence Signals with a Smartphone CMOS Sensor.
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DOI：
10.1021/acs.analchem.8b03521
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发表时间：
2018-11-06
影响因子：
7.4
通讯作者：
Meagher RJ
中科院分区：
文献类型：
--
作者：
Priye A;Ball CS;Meagher RJ
智能手机已经显示出作为便携式和分布式床旁诊断测试的使能技术的前景。CMOS相机传感器可用于检测光学信号，包括用于诸如等温核酸扩增测试等应用的荧光。然而，这种分析通常主要限于单个目标的端点检测。在这里，我们提出了一种基于智能手机的图像分析流水线，其利用CIE xyY（色度-亮度）颜色空间来测量由核酸扩增靶产生的荧光信号的亮度（代替RGB强度），其辨别灵敏度（正信号与负信号之间的比率）比传统的基于RGB强度的分析高一个数量级。此外，分析的色度部分能够可靠地多重检测用光谱分离的荧光团标记的不同目标。我们应用这种色度-亮度公式通过终点RT-LAMP（逆转录环介导等温扩增）同时检测寨卡病毒和基孔肯雅病毒RNA。我们还展示了在我们的智能手机操作的便携式LAMP盒中对淋病奈瑟菌样品的真实的实时LAMP检测，其拷贝数低至每10 μL反应体积3.5个拷贝。此外，色度-亮度分析很容易适用于使用智能手机相机的其他类型的多路复用荧光测量。使用智能手机摄像头实现多重荧光检测的高分辨力。
Smartphones have shown promise as an enabling technology for portable and distributed point-of-care diagnostic tests. The CMOS camera sensor can be used for detecting optical signals, including fluorescence for applications such as isothermal nucleic acid amplification tests. However, such analysis is typically limited mostly to endpoint detection of single targets. Here we present a smartphone-phone based image analysis pipeline that utilizes the CIE xyY (chromaticity-luminance) color space to measure the luminance (in lieu of RGB intensities) of fluorescent signals arising from nucleic acid amplification targets, with a discrimination sensitivity (ratio between the positive to negative signals) which is an order of magnitude more than traditional RGB intensity based analysis. Furthermore, the chromaticity part of the analysis enables reliable multiplexed detection of different targets labeled with spectrally separated fluorophores. We apply this chromaticity-luminance formulation to simultaneously detect Zika and chikungunya viral RNA via endpoint RT-LAMP (Reverse transcription Loop-Mediated isothermal amplification). We also show real time LAMP detection of Neisseria gonorrhoeae samples down to a copy number of 3.5 copies per 10 μL of reaction volume in our smartphone-operated portable LAMP box. Furthermore, the chromaticity-luminance analysis is readily adaptable to other types of multiplexed fluorescence measurements using a smartphone camera. High discrimination of multiplexed fluorescence assays using a smartphone camera.
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影响因子：
4.6
作者：
Knowlton SM;Sencan I;Aytar Y;Khoory J;Heeney MM;Ghiran IC;Tasoglu S
通讯作者：
Tasoglu S
影响因子：
7.4
作者：
Priye A;Wong S;Bi Y;Carpio M;Chang J;Coen M;Cope D;Harris J;Johnson J;Keller A;Lim R;Lu S;Millard A;Pangelinan A;Patel N;Smith L;Chan K;Ugaz VM
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Ugaz VM
影响因子：
6.1
作者：
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通讯作者：
Yang C
DOI：
10.1016/j.jpba.2016.03.018
发表时间：
2016-06-05
影响因子：
3.4
作者：
Yu, Hojeong;Le, Huy M.;Cunningham, Brian T.
通讯作者：
Cunningham, Brian T.
影响因子：
17.1
作者：
Wei, Qingshan;Qi, Hangfei;Luo, Wei;Tseng, Derek;Ki, So Jung;Wan, Zhe;Gorocs, Zoltan;Bentolila, Laurent A.;Wu, Ting-Ting;Sun, Ren;Ozcan, Aydogan
通讯作者：
Ozcan, Aydogan