Biomimetic heterogenous elastic tissue development.
Biomimetic heterogenous elastic tissue development.
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DOI：
10.1038/s41536-017-0021-4
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发表时间：
2017
影响因子：
7.2
通讯作者：
de Mel A
中科院分区：
文献类型：
--
作者：
Tsai KJ;Dixon S;Hale LR;Darbyshire A;Martin D;de Mel A
对于人造组织存在未满足的需求，以解决供体器官的当前限制和供体部位发病率的问题。尽管采用细胞作为构建模块的复杂组织工程努力取得了成功，但它们仅限于适合于细胞培养的专用实验室，并且在可用于临床使用之前具有相关的高成本和长的组织成熟时间。直接3D打印为颅骨和骨骼修复或置换提供了快速，定制，无细胞的解决方案，并且可以潜在地解决对弹性组织的需求，弹性组织是支持器官的平滑肌，软骨，韧带和结缔组织的主要组成部分。热塑性聚氨酯是最通用的弹性体聚合物之一。它们由硬链段和软链段组成的链段嵌段共聚物性质允许几乎无限的潜力来控制物理性能和机械行为。在这里，我们展示了使用熔融沉积建模直接3D打印生物相容性热塑性聚氨酯，以期呈现细胞独立的原位组织替代品。该方法可以快速且经济地产生具有受控孔隙率的异质仿生弹性组织替代物，以潜在地促进血管形成。此处以管状结构为例展示了该应用的灵活性。我们展示了这些3D打印结构如何进行后处理以整合生物活性分子。这种有效的策略，当与数字医疗的特权相结合时，可用于原位生产定制的弹性组织替代品，而不依赖于广泛的细胞培养，并可开发为即时治疗方法。无溶剂热塑性聚氨酯（TPU）可用于3D打印人造组织，节省时间和金钱。伦敦的Achala de Mel及其同事使用开源3D建模软件和商用3D打印机，用定制的TPU制造了一个定制的气管支架。该团队能够通过3D设计控制材料的孔隙率，如果植入，这可以促进其血管化。气管在机械和结构上与成人相似，表现出纵向弹性和径向刚性。当连接到呼吸机系统时，它对类似于吸气、用力呼气、咳嗽或哭泣的压力反应良好。3D打印的气管用生物活性分子处理，因此细胞可能会粘附在其表面并在其表面增殖。这种方法可以用来制造定制的弹性组织替代品，避免昂贵和耗时的细胞培养技术。
There is an unmet need for artificial tissue to address current limitations with donor organs and problems with donor site morbidity. Despite the success with sophisticated tissue engineering endeavours, which employ cells as building blocks, they are limited to dedicated labs suitable for cell culture, with associated high costs and long tissue maturation times before available for clinical use. Direct 3D printing presents rapid, bespoke, acellular solutions for skull and bone repair or replacement, and can potentially address the need for elastic tissue, which is a major constituent of smooth muscle, cartilage, ligaments and connective tissue that support organs. Thermoplastic polyurethanes are one of the most versatile elastomeric polymers. Their segmented block copolymeric nature, comprising of hard and soft segments allows for an almost limitless potential to control physical properties and mechanical behaviour. Here we show direct 3D printing of biocompatible thermoplastic polyurethanes with Fused Deposition Modelling, with a view to presenting cell independent in-situ tissue substitutes. This method can expeditiously and economically produce heterogenous, biomimetic elastic tissue substitutes with controlled porosity to potentially facilitate vascularisation. The flexibility of this application is shown here with tubular constructs as exemplars. We demonstrate how these 3D printed constructs can be post-processed to incorporate bioactive molecules. This efficacious strategy, when combined with the privileges of digital healthcare, can be used to produce bespoke elastic tissue substitutes in-situ, independent of extensive cell culture and may be developed as a point-of-care therapy approach. Solvent-free thermoplastic polyurethanes (TPU) could be used to 3D-print artificial tissues saving time and money. Achala de Mel and colleagues at University College London used open-source 3D-modelling software and commercially available 3D printers to fabricate a bespoke tracheal stent from custom-made TPU. The team was able to control the material’s porosity with 3D-design, which could facilitate its vascularisation if implanted. The trachea was mechanically and structurally similar to that of an adult, showing longitudinal elasticity and radial rigidity. When attached to a ventilator system, it responded well to pressures similar to those of inspiration, forced expiration, coughing or crying. 3D-printed trachea was treated with bioactive molecules so cells could potentially adhere to and proliferate on its surface. This method could be used to fabricate bespoke elastic tissue substitutes, avoiding costly and time-consuming cell-culture techniques.
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影响因子：
16.6
作者：
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通讯作者：
Cho DW
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影响因子：
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作者：
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通讯作者：
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