Surveillance of Aedes aegypti populations in the city of Praia, Cape Verde: Zika virus infection, insecticide resistance and genetic diversity.
Surveillance of Aedes aegypti populations in the city of Praia, Cape Verde: Zika virus infection, insecticide resistance and genetic diversity.
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DOI：
10.1186/s13071-020-04356-z
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发表时间：
2020-09-21
影响因子：
3.2
通讯作者：
Campino S
中科院分区：
文献类型：
--
作者：
Campos M;Ward D;Morales RF;Gomes AR;Silva K;Sepúlveda N;Gomez LF;Clark TG;Campino S
伊蚊是许多虫媒病毒的传播媒介，导致世界范围内人类发病率和死亡率上升。埃及伊蚊是基孔肯雅热、登革热和黄热病感染的主要媒介，其发病率不断上升，分布不断扩大。这一病媒还推动了寨卡病毒（ZIKV）的出现，寨卡病毒首先在非洲报告，随后迅速传播到亚洲，最近又蔓延到整个美洲。在美洲爆发寨卡疫情期间，佛得角成为第一个宣布出现寨卡疫情的非洲国家，确诊了小头症病例。在此，我们调查了寨卡病毒和登革热（DENV）感染伊蚊的流行情况。佛得角暴发后数周内的埃及伊蚊，以及传播媒介种群中存在的杀虫剂耐药性。还分析了蚊子种群的遗传多样性。2016年8月至10月，816 Ae。在佛得角普拉亚的几个地点采集了埃及伊蚊，普拉亚是该国报告的寨卡病毒病例的主要热点。采用反转录PCR方法对所有蚊子进行寨卡病毒和DENV病毒的检测，并通过毛细管测序对220只蚊子进行电压门控钠通道（VGSC）基因抗虫相关突变的检测。对100只蚊子线粒体NADH脱氢酶亚基4 （nad4）基因进行了测序。这些数据通过单倍型网络和系统发育树分析与977个全球序列进行了比较。两个Ae。埃及伊蚊寨卡病毒阳性（0.25%）。VGSC基因中未发现与杀虫剂抗性相关的SNP突变。对nad4基因的分析显示，佛得角样本中有11个单倍型，其中5个为单倍型。7个单倍型是佛得角独有的。剩下的几个单倍型在全球数据集中很常见，存在于五大洲的几个国家（包括佛得角）。佛得角最常见的单倍型（50.6%）也在非洲和南美洲发现。疫情爆发后不久，来自普拉亚的蚊子中出现了低水平的寨卡病毒传播。Ae。埃及伊蚊种群似乎没有与拟除虫菊酯类杀虫剂抗性相关的KDR突变。此外，单倍型和系统发育分析表明，佛得角Ae。埃及伊蚊与来自非洲和南美洲其他国家的蚊子关系最为密切。
Aedes spp. are responsible for the transmission of many arboviruses, which contribute to rising human morbidity and mortality worldwide. The Aedes aegypti mosquito is a main vector for chikungunya, dengue and yellow fever infections, whose incidence have been increasing and distribution expanding. This vector has also driven the emergence of the Zika virus (ZIKV), first reported in Africa which spread rapidly to Asia and more recently across the Americas. During the outbreak in the Americas, Cape Verde became the first African country declaring a Zika epidemic, with confirmed cases of microcephaly. Here we investigate the prevalence of ZIKV and dengue (DENV) infected Ae. aegypti mosquitoes in the weeks following the outbreak in Cape Verde, and the presence of insecticide resistance in the circulating vector population. Genetic diversity in the mosquito population was also analysed. From August to October 2016, 816 Ae. aegypti mosquitoes were collected in several locations across Praia, Cape Verde, the major hot spot of reported ZIKV cases in the country. All mosquitoes were screened by reverse transcription PCR for ZIKV and DENV, and a subset (n = 220) were screened for knockdown insecticide resistance associated mutations in the voltage gated sodium channel (VGSC) gene by capillary sequencing. The mitochondrial NADH dehydrogenase subunit 4 (nad4) gene was sequenced in 100 mosquitoes. These data were compared to 977 global sequences in a haplotype network and a phylogenetic tree analysis. Two Ae. aegypti mosquitoes were ZIKV positive (0.25%). There were no SNP mutations found in the VGSC gene associated with insecticide resistance. Analysis of the nad4 gene revealed 11 haplotypes in the Cape Verdean samples, with 5 being singletons. Seven haplotypes were exclusive to Cape Verde. Several of the remaining haplotypes were frequent in the global dataset, being present in several countries (including Cape Verde) across five different continents. The most common haplotype in Cape Verde (50.6 %) was also found in Africa and South America. There was low-level Zika virus circulation in mosquitoes from Praia shortly after the outbreak. The Ae. aegypti population did not appear to have the kdr mutations associated with pyrethroid resistance. Furthermore, haplotype and phylogenetic analyses revealed that Cape Verde Ae. aegypti mosquitoes are most closely related to those from other countries in Africa and South America.
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影响因子：
3.2
作者：
Dia, Ibrahima;Diagne, Cheikh Tidiane;Diallo, Mawlouth
通讯作者：
Diallo, Mawlouth
基因组流行病学揭示了寨卡病毒的多次引入到美国。
影响因子：
64.8
作者：
Grubaugh ND;Ladner JT;Kraemer MUG;Dudas G;Tan AL;Gangavarapu K;Wiley MR;White S;Thézé J;Magnani DM;Prieto K;Reyes D;Bingham AM;Paul LM;Robles-Sikisaka R;Oliveira G;Pronty D;Barcellona CM;Metsky HC;Baniecki ML;Barnes KG;Chak B;Freije CA;Gladden-Young A;Gnirke A;Luo C;MacInnis B;Matranga CB;Park DJ;Qu J;Schaffner SF;Tomkins-Tinch C;West KL;Winnicki SM;Wohl S;Yozwiak NL;Quick J;Fauver JR;Khan K;Brent SE;Reiner RC Jr;Lichtenberger PN;Ricciardi MJ;Bailey VK;Watkins DI;Cone MR;Kopp EW 4th;Hogan KN;Cannons AC;Jean R;Monaghan AJ;Garry RF;Loman NJ;Faria NR;Porcelli MC;Vasquez C;Nagle ER;Cummings DAT;Stanek D;Rambaut A;Sanchez-Lockhart M;Sabeti PC;Gillis LD;Michael SF;Bedford T;Pybus OG;Isern S;Palacios G;Andersen KG
通讯作者：
Andersen KG
影响因子：
4.9
作者：
Bennett, Kelly Louise;Shija, Fortunate;Walton, Catherine
通讯作者：
Walton, Catherine
影响因子：
1.9
作者：
Brengues, C;Hawkes, NJ;Hemingway, J
通讯作者：
Hemingway, J
影响因子：
3
作者：
da Cruz, Derciliano Lopes;Santos Paiva, Marcelo Henrique;Junqueira Ayres, Constancia Flavia
通讯作者：
Junqueira Ayres, Constancia Flavia