Permeability of the rhesus monkey oocyte membrane to water and common cryoprotectants.
Permeability of the rhesus monkey oocyte membrane to water and common cryoprotectants.
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DOI：
10.1002/mrd.20956
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发表时间：
2009-04
影响因子：
2.5
通讯作者：
Eroglu, Ali
中科院分区：
文献类型：
--
作者：
Karlsson, Jens O. M.;Younis, Abdelmoneim I.;Chan, Anthony W. S.;Gould, Kenneth G.;Eroglu, Ali
恒河猴卵母细胞冷冻保存的成功将有助于这一宝贵的动物模型在生殖和发育研究中的应用，同时也为人类卵母细胞冷冻保存和濒危灵长类动物的保护提供了一个垫脚石。为了合理设计恒河猴卵母细胞的冷冻保存技术，我们已经确定了它们的渗透和渗透特性的存在下，二甲基亚砜（DMSO），乙二醇（EG），丙二醇（PROH），三个广泛使用的冷冻保护剂。使用非线性回归拟合膜运输模型来测量动态细胞体积变化，我们估计了23.5°C下成熟和未成熟卵母细胞的水力传导率（Lp）和冷冻保护剂渗透率（Ps）。成熟卵母细胞膜对PROH的渗透性最高（Ps = 0.56 ± 0.05 µm/sec），对DMSO的渗透性最低（Ps = 0.24 ± 0.02 µm/sec）;对EG的渗透性为0.34 ± 0.07 µm/sec。在没有渗透性冷冻保护剂的情况下，成熟卵母细胞的Lp = 0.55 ± 0.05 µm/min/atm，而当成熟卵母细胞暴露于DMSO、EG或PROH时，水力传导率分别增加至1.01 ± 0.10、0.61 ± 0.07或0.86 ± 0.06 µm/min/atm。成熟卵母细胞的无促排卵活性体积（Vb）为等渗细胞体积的19.7 ± 2.4%。成熟卵母细胞和未成熟卵母细胞之间唯一的统计学显著差异是暴露于DMSO的未成熟卵母细胞的水力传导率较大。在这项研究中测量的生物物理参数被用来证明冷冻保护剂加载和稀释方案的设计，通过计算机辅助优化。
Successful cryopreservation of oocytes of the rhesus monkey (Macaca mulatta) would facilitate the use of this valuable animal model in research on reproduction and development, while providing a stepping stone towards human oocyte cryopreservation and the conservation of endangered primate species. To enable rational design of cryopreservation techniques for rhesus monkey oocytes, we have determined their osmotic and permeability characteristics in the presence of dimethylsulfoxide (DMSO), ethylene glycol (EG), and propylene glycol (PROH), three widely used cryoprotectants. Using nonlinear regression to fit a membrane transport model to measurements of dynamic cell volume changes, we estimated the hydraulic conductivity (Lp) and cryoprotectant permeability (Ps) of mature and immature oocytes at 23.5°C. Mature oocyte membranes were most permeable to PROH (Ps = 0.56 ± 0.05 µm/sec) and least permeable to DMSO (Ps = 0.24 ± 0.02 µm/sec); the permeability to EG was 0.34 ± 0.07 µm/sec. In the absence of penetrating cryoprotectants, mature oocytes had Lp = 0.55 ± 0.05 µm/min/atm, whereas the hydraulic conductivity increased to 1.01 ± 0.10, 0.61 ± 0.07, or 0.86 ± 0.06 µm/min/atm when mature oocytes were exposed to DMSO, EG, or PROH, respectively. The osmotically inactive volume (Vb) in mature oocytes was19.7 ± 2.4% of the isotonic cell volume. The only statistically significant difference between mature and immature oocytes was a larger hydraulic conductivity in immature oocytes that were exposed to DMSO. The biophysical parameters measured in this study were used to demonstrate the design of cryoprotectant loading and dilution protocols by computer-aided optimization.
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影响因子：
2.7
作者：
ARNAUD, FG;PEGG, DE
通讯作者：
PEGG, DE
影响因子：
6.1
作者：
ASHWOODSMITH, MJ;MORRIS, GW;ASHORN, R
通讯作者：
ASHORN, R
影响因子：
2.4
作者：
LEIBO, SP
通讯作者：
LEIBO, SP
影响因子：
6.1
作者：
Kuleshova, L;Gianaroli, L;Trounson, A
通讯作者：
Trounson, A
DOI：
10.1530/jrf.0.0900547
发表时间：
1990-11-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
CARROLL, J;DEPYPERE, H;MATTHEWS, CD
通讯作者：
MATTHEWS, CD