Strategies to prevent hydrolytic degradation of the hybrid layer-A review.
Strategies to prevent hydrolytic degradation of the hybrid layer-A review.
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DOI：
10.1016/j.dental.2013.07.016
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发表时间：
2013-10
期刊：
影响因子：
5
通讯作者：
Pashley, David H.
中科院分区：
文献类型：
--
作者：
Tjaderhane, Leo;Nascimento, Fabio D.;Breschi, Lorenzo;Mazzoni, Annalisa;Tersariol, Ivarne L. S.;Geraldeli, Saulo;Tezvergil-Mutluay, Arzu;Carrilho, Marcela;Carvalho, Ricardo M.;Tay, Franklin R.;Pashley, David H.
关键词：
内源性牙本质胶原降解酶，基质金属蛋白酶（MMPs）和半胱氨酸组织蛋白酶，负责与时间相关的混合层的胶原基质的水解。由于胶原基质的完整性对于保持长期牙本质粘结强度至关重要，因此抑制或灭活内源性牙本质蛋白酶对于持久的树脂粘结复合树脂粘结是必要的。牙本质中含有胶原分解酶、基质金属蛋白酶和半胱氨酸组织蛋白酶，它们负责在粘结界面中水解降解胶原基质。文献中已经提出了几种直接或间接阻止酶功能的尝试性方法。洗必泰是一种MMP和半胱氨酸组织蛋白酶的一般抑制剂，在底漆/粘合剂应用之前应用是最受测试的方法。一般来说，这些实验表明，酶抑制是一个有前途的计划，以改善混合层的保存和粘结强度耐久性。其他酶抑制剂，例如酶抑制单体和抗微生物化合物，可能被认为是有前景的替代品，其将允许比氯己定更简单的临床应用。交联胶原蛋白和/或牙本质有机基质结合酶可以使混合层有机基质抗降解，并且用乙醇湿粘合或仿生生物矿化从混合层中完全去除水应该消除胶原蛋白和树脂组分的水解。鉴定负责水解混合层胶原蛋白的酶并了解其功能，促使了几种创新方法来保持混合层的完整性和强牙本质结合。最终目标，即利用在临床环境中简单有效的技术和市售材料预防胶原基质降解，可以通过多种方式实现，并可能在不久的将来成为现实。
Endogenous dentin collagenolytic enzymes, matrix metalloproteinases (MMPs) and cysteine cathepsins, are responsible for the time-related hydrolysis of collagen matrix of the hybrid layers. As the integrity of the collagen matrix is essential for the preservation of long-term dentin bond strength, inhibition or inactivation of endogenous dentin proteases is necessary for durable resin-bonded composite resin restorations. Dentin contains collagenolytic enzymes, matrix metalloproteinases (MMPs) and cysteine cathepsins, which are responsible for the hydrolytic degradation of collagen matrix in the bonded interface. Several tentative approaches to prevent enzyme function either directly or indirectly have been proposed in the literature. Chlorhexidine, a general inhibitor of both MMPs and cysteine cathepsins, applied before primer/adhesive application is the most tested method. In general, these experiments have shown that enzyme inhibition is a promising scheme to improve hybrid layer preservation and bond strength durability. Other enzyme inhibitors, e.g. enzyme-inhibiting monomers and antimicrobial compounds, may be considered promising alternatives that would allow more simple clinical application than chlorhexidine. Cross-linking collagen and/or dentin organic matrix-bound enzymes could render hybrid layer organic matrix resistant to degradation, and complete removal of water from the hybrid layer with ethanol wet bonding or biomimetic remineralization should eliminate hydrolysis of both collagen and resin components. Identification of the enzymes responsible for the hydrolysis of hybrid layer collagen and understanding their function has prompted several innovative approaches to retain the hybrid layer integrity and strong dentin bonding. The ultimate goal, prevention of collagen matrix degradation with techniques and commercially available materials that are simple and effective in clinical settings may be achievable in several ways, and will likely become reality in the near future.
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影响因子：
7.6
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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影响因子：
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