Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma.
Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma.
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DOI：
10.1186/1471-2407-5-119
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发表时间：
2005-09-20
期刊：
影响因子：
3.8
通讯作者：
Stephenson SA
中科院分区：
文献类型：
--
作者：
Lee YC;Perren JR;Douglas EL;Raynor MP;Bartley MA;Bardy PG;Stephenson SA
据报道，EphB4受体酪氨酸激酶在起源于几个不同组织的肿瘤中增加，其在前列腺癌异种移植模型中的表达已被报道。应用RT-PCR、Western blotting和免疫组织化学技术检测EphB4在人前列腺癌LNCaP、DU145和PC3细胞中的表达和蛋白水平。应用免疫组织化学方法检测15例前列腺癌组织标本中EphB4的定位。三种前列腺癌细胞株都表达EphB4基因和蛋白。在前列腺癌组织中，EphB4的免疫反应性明显高于匹配的正常前列腺上皮组织，并且随着疾病级别的增加，EphB4的表达有增加的趋势。EphB4在前列腺癌细胞系中表达，与匹配的正常组织相比，在人类前列腺癌中的表达增加。因此，EphB4可能是一个有用的抗前列腺癌靶点。
The EphB4 receptor tyrosine kinase has been reported as increased in tumours originating from several different tissues and its expression in a prostate cancer xenograft model has been reported. RT-PCR, western blotting and immunohistochemical techniques were used to examine EphB4 expression and protein levels in human prostate cancer cell lines LNCaP, DU145 and PC3. Immunohistochemistry was also used to examine localisation of EphB4 in tissue samples from 15 patients with prostate carcinomas. All three prostate cancer cell lines expressed the EphB4 gene and protein. EphB4 immunoreactivity in vivo was significantly greater in human prostate cancers as compared with matched normal prostate epithelium and there appeared to be a trend towards increased expression with higher grade disease. EphB4 is expressed in prostate cancer cell lines with increased expression in human prostate cancers when compared with matched normal tissue. EphB4 may therefore be a useful anti-prostate cancer target.
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DOI：
10.1006/bbrc.1996.1442
发表时间：
1996-09-24
影响因子：
3.1
作者：
Berclaz, G;Andres, AC;Crompton, MR
通讯作者：
Crompton, MR
影响因子：
6.2
作者：
Liu, WB;Ahmad, SA;Ellis, LM
通讯作者：
Ellis, LM
影响因子：
--
作者：
Stephenson, S A;Slomka, S;Hardingham, J E
通讯作者：
Hardingham, J E
影响因子：
50.5
作者：
Berclaz, G;Karamitopoulou, E;Andres, AC
通讯作者：
Andres, AC
影响因子：
64.5
作者：
Wang, HU;Chen, ZF;Anderson, DJ
通讯作者：
Anderson, DJ