Hidden in plain sight - Multiple resistant species within a strongyle community.
Hidden in plain sight - Multiple resistant species within a strongyle community.
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DOI：
10.1016/j.vetpar.2018.06.012
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发表时间：
2018-07-15
影响因子：
2.6
通讯作者：
Laing R
中科院分区：
文献类型：
--
作者：
McIntyre J;Hamer K;Morrison AA;Bartley DJ;Sargison N;Devaney E;Laing R
聚合酶链式反应的物种多样性突出了英国一个商业养羊场的寄生虫物种多样性。物种多样性混淆了对粪便卵数数据和生物测定的解释。这些检测只检测到对苯并咪唑和伊维菌素的中等耐药性。处理后的种群几乎全部由环斑天牛组成。伊维菌素强烈被选为高度双重抗性和致病的物种。绵羊寄生虫性胃肠炎是一种复杂的疾病，通常使用驱虫药进行治疗。虽然许多不同的种可能导致寄生性胃肠炎，但并不是所有种的致病性都是一样的：在温带地区，主要病原体是环状丝虫。在这项研究中，我们调查了苏格兰东南部一个商业羊场对苯并咪唑和伊维菌素的耐药性。我们通过粪卵计数减少试验和体外生物测定，评估了物种多样性对耐药性诊断的影响，并将结果与通过β-微管蛋白同型1 基因焦磷酸测序测定的苯并咪唑耐药性相关基因在圆锥虫种群中的频率相关联。粪便减蛋试验结果表明，阿苯达唑的有效率为65%，伊维菌素的有效率为77%，表明该养殖场的抗性水平为中等。然而，相同种群处理前后的聚合酶链式反应结果显示，去除敏感种群掩盖了高抗性种群的存在。在处理前的种群中，只有不到25%的个体是圆锥虫，其余的个体包括古柯贝、羊轮枝藻、脉管食道口线虫和毛圆线虫。相比之下，使用阿苯达唑或伊维菌素治疗后，大多数(分别为88%和100%)的种群由周毛虫组成。对同一种群的虫卵进行了对苯并咪唑抗性的卵孵化试验和对伊维菌素抗性的幼虫发育试验，结果与粪卵计数减少试验基本一致。来自每个采样时间点的30个圆锥虫个体通过焦磷酸测序评估了苯并咪唑的耐药性，揭示了包括在伊维菌素治疗后采样的人群中的耐药相关突变的高频和多样性。这些结果突显了英国农场存在的寄生虫物种的潜在多样性，以及它们在诊断驱虫药耐药性方面的重要性。在这个特殊的农场上，我们证明了存在高度双重抗性的圆锥虫种群，该种群通过苯并咪唑或伊维菌素处理而被强烈选择，而其他潜在的较低致病性物种被移除。
PCR speciation highlighted parasite species diversity on a commercial UK sheep farm. Species diversity confounded interpretation of faecal egg count data and bioassays. These tests detected only moderate resistance to benzimidazoles and ivermectin. Post-treatment populations were composed almost entirely of Teladorsagia circumcincta. Ivermectin strongly selected for a highly dual-resistant and pathogenic species. Ovine parasitic gastroenteritis is a complex disease routinely treated using anthelmintics. Although many different strongyle species may contribute to parasitic gastroenteritis, not all are equally pathogenic: in temperate regions, the primary pathogen is Teladorsagia circumcincta. In this study we investigated benzimidazole and ivermectin resistance on a commercial sheep farm in southeast Scotland. We assessed the impact of species diversity on the diagnosis of resistance using the faecal egg count reduction test and in vitro bioassays, and correlated the results with the frequency of benzimidazole resistance-associated genotypes measured in the T. circumcincta population by pyrosequencing of the β-tubulin isotype-1 gene. Faecal egg count reduction test results showed efficacies of 65% for albendazole and 77% for ivermectin, indicating moderate resistance levels on the farm. However, PCR speciation of the same populations pre- and post-treatment revealed that removal of susceptible species had masked the presence of a highly resistant population of T. circumcincta. Less than 25% of individuals in the pre-treatment populations were T. circumcincta, the remainder consisting of Cooperia curticei, Chabertia ovina, Oesophagostomum venulosum and Trichostrongylus spp. In contrast, post-treatment with albendazole or ivermectin, the majority (88% and 100% respectively) of the populations consisted of T. circumcincta. The egg hatch test for benzimidazole resistance and the larval development test for ivermectin resistance were carried out using eggs obtained from the same populations and the results were broadly consistent with the faecal egg count reduction test. Thirty individual T. circumcincta from each sampling time point were assessed for benzimidazole resistance by pyrosequencing, revealing a high frequency and diversity of resistance-associated mutations, including within the population sampled post-ivermectin treatment. These results highlight the potential diversity of parasite species present on UK farms, and their importance in the diagnosis of anthelmintic resistance. On this particular farm, we demonstrate the presence of a highly dual-resistant population of T. circumcincta, which was strongly selected by treatment with either benzimidazoles or ivermectin, while other potentially less pathogenic species were removed.
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影响因子：
1.1
作者：
MARTIN, PJ;ANDERSON, N;JARRETT, RG
通讯作者：
JARRETT, RG
DOI：
10.1016/j.ijpddr.2014.07.007
发表时间：
2014-12
影响因子：
4
作者：
Kotze, Andrew C.;Hunt, Peter W.;Skuce, Philip;von Samson-Himmelstjerna, Georg;Martin, Richard J.;Sager, Heinz;Kruecken, Juergen;Hodgkinson, Jane;Lespine, Anne;Jex, Aaron R.;Gilleard, John S.;Beech, Robin N.;Wolstenholme, Adrian J.;Demeler, Janina;Robertson, Alan P.;Charvet, Claude L.;Neveu, Cedric;Kaminsky, Ronald;Rufener, Lucien;Alberich, Melanie;Menez, Cecile;Prichard, Roger K.
通讯作者：
Prichard, Roger K.
影响因子：
2.6
作者：
Learmount, Jane;Gettinby, George;Taylor, Mike
通讯作者：
Taylor, Mike
DOI：
10.1016/j.ijpddr.2013.04.002
发表时间：
2013-12
影响因子：
4
作者：
Demeler, Janina;Gill, Jennifer H.;von Samson-Himmelstjerna, Georg;Sangster, Nicholas C.
通讯作者：
Sangster, Nicholas C.
影响因子：
2.2
作者：
KERBOEUF, D;HUBERT, J;BLONDRIOU, F
通讯作者：
BLONDRIOU, F