Absence of evidence of xenotropic murine leukemia virus-related virus infection in persons with chronic fatigue syndrome and healthy controls in the United States.
Absence of evidence of xenotropic murine leukemia virus-related virus infection in persons with chronic fatigue syndrome and healthy controls in the United States.
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DOI：
10.1186/1742-4690-7-57
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发表时间：
2010-07-01
期刊：
影响因子：
3.3
通讯作者：
Heneine W
中科院分区：
文献类型：
--
作者：
Switzer WM;Jia H;Hohn O;Zheng H;Tang S;Shankar A;Bannert N;Simmons G;Hendry RM;Falkenberg VR;Reeves WC;Heneine W
XMRV是一种异嗜性小鼠白血病病毒(MuLV)相关病毒，最近在67%的慢性疲劳综合征(CFS)患者和3.7%的美国健康人中被检测出XMRV。为了探讨XMRV与CFS的关系，我们用血清学和分子检测方法检测了51例CFS患者和56例美国健康人的血液标本，以寻找XMRV感染的证据。在美国疾病控制和预防中心(CDC)和另外两个实验室进行了盲法PCR和血清学测试。来自1994年国际研究病例定义的CFS患者和来自堪萨斯州威奇托以及佐治亚州大都市、城市和农村人口的健康对照的存档血液样本进行了测试。CDC的血清学检测采用了西方印迹(WB)分析，该分析对MuLV和XMRV多克隆或单克隆抗体具有极好的敏感性，对121名美国献血者或26份HTLV和HIV感染血清没有反应。51例CFS患者和53例正常对照血浆均为WB阴性。Robert Koch研究所使用重组Gag和Env XMRV蛋白对51例患者和53名对照进行了额外的盲法筛查，发现1例CFS患者和1名健康对照的血清反应较弱，这一点未经免疫荧光证实。疾控中心的聚合酶链式反应检测采用GAG和POL套式聚合酶链式反应，检测阈值为1微克人DNA中有10个拷贝。50例CFS患者、56例对照组和41例美国献血员的DNA标本均为阴性。血液系统研究所的第二次套式GAG PCR盲法检测50例CFS患者和56例对照的DNA样本也为阴性。通过多种分子和血清学分析，我们在美国研究人群中没有发现任何感染XMRV的证据，即CFS患者或健康对照。这些数据不支持XMRV与CFS的关联。
XMRV, a xenotropic murine leukemia virus (MuLV)-related virus, was recently identified by PCR testing in 67% of persons with chronic fatigue syndrome (CFS) and in 3.7% of healthy persons from the United States. To investigate the association of XMRV with CFS we tested blood specimens from 51 persons with CFS and 56 healthy persons from the US for evidence of XMRV infection by using serologic and molecular assays. Blinded PCR and serologic testing were performed at the US Centers for Disease Control and Prevention (CDC) and at two additional laboratories. Archived blood specimens were tested from persons with CFS defined by the 1994 international research case definition and matched healthy controls from Wichita, Kansas and metropolitan, urban, and rural Georgia populations. Serologic testing at CDC utilized a Western blot (WB) assay that showed excellent sensitivity to MuLV and XMRV polyclonal or monoclonal antibodies, and no reactivity on sera from 121 US blood donors or 26 HTLV-and HIV-infected sera. Plasma from 51 CFS cases and plasma from 53 controls were all WB negative. Additional blinded screening of the 51 cases and 53 controls at the Robert Koch Institute using an ELISA employing recombinant Gag and Env XMRV proteins identified weak seroreactivity in one CFS case and a healthy control, which was not confirmed by immunofluorescence. PCR testing at CDC employed a gag and a pol nested PCR assay with a detection threshold of 10 copies in 1 ug of human DNA. DNA specimens from 50 CFS patients and 56 controls and 41 US blood donors were all PCR-negative. Blinded testing by a second nested gag PCR assay at the Blood Systems Research Institute was also negative for DNA specimens from the 50 CFS cases and 56 controls. We did not find any evidence of infection with XMRV in our U.S. study population of CFS patients or healthy controls by using multiple molecular and serologic assays. These data do not support an association of XMRV with CFS.
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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作者：
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影响因子：
3.3
作者：
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