Role of sensory experience in functional development of Drosophila motor circuits.
Role of sensory experience in functional development of Drosophila motor circuits.
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DOI：
10.1371/journal.pone.0062199
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nose A
中科院分区：
文献类型：
--
作者：
Fushiki A;Kohsaka H;Nose A
神经元回路是根据基因预定的程序形成的，然后以依赖经验的方式重建。虽然在视觉和其他感觉系统中已经证明了经验依赖性可塑性的存在，但对于运动系统是否也是如此仍然未知。在这里，我们研究了消除感觉输入对果蝇胚胎和幼虫蠕动运动发育的影响。胚胎发育最初缓慢且不协调，但在胚胎后期逐渐发展成成熟模式。我们测试了在此期间抑制特定感觉神经元的传递是否会对感觉运动回路的特性产生持久影响。我们在胚胎发育期间（产卵后15-21小时[AEL]）应用了6小时的Shibire介导的抑制，并研究了其对成熟的第二和第三龄幼虫中的寄生虫病的影响。我们发现，抑制弦音器官，但不是多树突神经元，导致幼虫运动的速度持久下降。为了缩小敏感期，我们在不同的胚胎和幼虫阶段应用了较短的抑制，发现在16-20 h的AEL期间，而不是在早期或后期阶段，两个小时的抑制就足以引起效果。这些结果表明，由特定的感觉神经元介导的神经活动参与了果蝇感觉运动回路的成熟，并且这种可塑性变化存在一个关键时期。与弦音神经元在感觉反馈中的作用相一致，这些神经元在幼虫期被激活，急性抑制它们的活性会降低幼虫运动的速度。
Neuronal circuits are formed according to a genetically predetermined program and then reconstructed in an experience-dependent manner. While the existence of experience-dependent plasticity has been demonstrated for the visual and other sensory systems, it remains unknown whether this is also the case for motor systems. Here we examined the effects of eliminating sensory inputs on the development of peristaltic movements in Drosophila embryos and larvae. The peristalsis is initially slow and uncoordinated, but gradually develops into a mature pattern during late embryonic stages. We tested whether inhibiting the transmission of specific sensory neurons during this period would have lasting effects on the properties of the sensorimotor circuits. We applied Shibire-mediated inhibition for six hours during embryonic development (15–21 h after egg laying [AEL]) and studied its effects on peristalsis in the mature second- and third-instar larvae. We found that inhibition of chordotonal organs, but not multidendritic neurons, led to a lasting decrease in the speed of larval locomotion. To narrow down the sensitive period, we applied shorter inhibition at various embryonic and larval stages and found that two-hour inhibition during 16–20 h AEL, but not at earlier or later stages, was sufficient to cause the effect. These results suggest that neural activity mediated by specific sensory neurons is involved in the maturation of sensorimotor circuits in Drosophila and that there is a critical period for this plastic change. Consistent with a role of chordotonal neurons in sensory feedback, these neurons were activated during larval peristalsis and acute inhibition of their activity decreased the speed of larval locomotion.
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影响因子：
5.5
作者：
HUBEL, DH;WIESEL, TN
通讯作者：
WIESEL, TN
影响因子：
64.8
作者：
Suster, ML;Bate, M
通讯作者：
Bate, M
DOI：
10.1111/j.1460-9568.2012.08075.x
发表时间：
2012-05
期刊：
The European journal of neuroscience
影响因子：
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作者：
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通讯作者：
Gaspar P
DOI：
10.1002/neu.10029
发表时间：
2002-02-15
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
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作者：
Keller, A;Sweeney, ST;Heisenberg, M
通讯作者：
Heisenberg, M
DOI：
10.1073/pnas.0700895104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Song, Wei;Onishi, Maika;Jan, Yuh Nung
通讯作者：
Jan, Yuh Nung