Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue.
Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue.
复制标题
心包和皮下脂肪组织的模式规范和免疫反应转录特征。
DOI：
10.1371/journal.pone.0026092
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Cowan CA
中科院分区：
文献类型：
--
作者：
Lau FH;Deo RC;Mowrer G;Caplin J;Ahfeldt T;Kaplan A;Ptaszek L;Walker JD;Rosengard BR;Cowan CA
心血管疾病（CVD）仍然是美国发病率和死亡率的主要原因。最近的研究表明，心包脂肪组织（PCAT）分泌的炎症因子，有助于CVD的发展。为了更好地表征PCAT在疾病发病机制中的作用，我们对PCAT和皮下脂肪组织之间的转录差异进行了大规模无偏分析，分析了19个个体的53个微阵列。由于不知道PCAT分泌的因子是由脂肪细胞还是由支持基质部分中的细胞产生，我们还试图鉴定分离的心包脂肪细胞与分离的皮下脂肪细胞中的差异表达基因。使用微阵列分析，我们发现：1）心包脂肪组织和分离的心包脂肪细胞都过表达促进动脉粥样硬化的趋化因子，2）心包和皮下脂肪库，以及分离的心包脂肪细胞和皮下脂肪细胞，表达同源异型盒基因的特定模式。与此相反，一组核心的脂质加工基因没有表现出显着的重叠与差异表达的转录本。这些库特异性同源框签名和转录谱强烈表明心包和皮下脂肪库的不同功能作用。进一步表征这些仓库间的差异应该是一个研究的优先事项。
Cardiovascular disease (CVD) remains the leading cause of morbidity and mortality in the United States. Recent studies suggest that pericardial adipose tissue (PCAT) secretes inflammatory factors that contribute to the development of CVD. To better characterize the role of PCAT in the pathogenesis of disease, we performed a large-scale unbiased analysis of the transcriptional differences between PCAT and subcutaneous adipose tissue, analysing 53 microarrays across 19 individuals. As it was unknown whether PCAT-secreted factors are produced by adipocytes or cells in the supporting stromal fraction, we also sought to identify differentially expressed genes in isolated pericardial adipocytes vs. isolated subcutaneous adipocytes. Using microarray analysis, we found that: 1) pericardial adipose tissue and isolated pericardial adipocytes both overexpress atherosclerosis-promoting chemokines and 2) pericardial and subcutaneous fat depots, as well as isolated pericardial adipocytes and subcutaneous adipocytes, express specific patterns of homeobox genes. In contrast, a core set of lipid processing genes showed no significant overlap with differentially expressed transcripts. These depot-specific homeobox signatures and transcriptional profiles strongly suggest different functional roles for the pericardial and subcutaneous adipose depots. Further characterization of these inter-depot differences should be a research priority.
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DOI：
10.1073/pnas.0601752103
发表时间：
2006-04-25
影响因子：
11.1
作者：
Gesta, S;Blüher, M;Kahn, CR
通讯作者：
Kahn, CR
影响因子：
24
作者：
Ford, Earl S.;Capewell, Simon
通讯作者：
Capewell, Simon
影响因子：
9.3
作者：
Baker, Adam R.;da Silva, Nancy F.;Quinn, David W.;Harte, Alison L.;Pagano, Domenico;Bonser, Robert S.;Kumar, Sudhesh;McTernan, Philip G.
通讯作者：
McTernan, Philip G.
影响因子：
6.9
作者：
Ding, Jingzhong;Kritchevsky, Stephen B.;Carr, J. Jeffrey
通讯作者：
Carr, J. Jeffrey
影响因子：
4.9
作者：
Cheng, K-H;Chu, C-S;Lai, W-T
通讯作者：
Lai, W-T