Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.
Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.
复制标题
DOI：
10.1093/pcp/pct133
复制
发表时间：
2013-11
影响因子：
4.9
通讯作者：
Watanabe M
中科院分区：
文献类型：
--
作者：
Osaka M;Matsuda T;Sakazono S;Masuko-Suzuki H;Maeda S;Sewaki M;Sone M;Takahashi H;Nakazono M;Iwano M;Takayama S;Shimizu KK;Yano K;Lim YP;Suzuki G;Suwabe K;Watanabe M
授粉是植物繁殖的早期和关键步骤，导致成功受精。它包括许多连续的过程，包括花粉粒粘附在柱头乳突细胞表面，足的形成，加强花粉-柱头的相互作用，花粉水合和萌发，以及花粉管的伸长和穿透。我们集中在乳突细胞中表达的基因的检查，以增加对授粉的分子系统的理解。从拟南芥科（Arabidopsis thaliana，A. halleri和芜菁），通过激光显微切割精确分离柱头乳突细胞，并通过RNA测序确定乳突细胞中细胞类型特异性基因表达。结果，A的乳头细胞中定义了17，240个、19，260个和21，026个单基因。thaliana，A. halleri和B. rapa，其中12，311个基因为所有三个物种所共有。在A.在拟南芥中，三分之一是乳头特异性的，而在所检查的所有组织中检测到大约一半的基因。生物信息学分析表明，与多种生殖和发育功能相关的基因在乳头细胞中表达，特别是代谢、转录和膜介导的信息交换。这些结果反映了一般细胞功能的保守特征以及乳头细胞的特定生殖作用，突出了这些细胞中由多种分子调控的复杂细胞系统。本研究为揭示乳突细胞授粉的分子机制提供了基础性的生物学知识，并将有助于我们对植物生殖机制的理解。
Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen–stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.
登录
查看更多内容
影响因子：
4.9
作者：
Goto H;Okuda S;Mizukami A;Mori H;Sasaki N;Kurihara D;Higashiyama T
通讯作者：
Higashiyama T
影响因子：
9.4
作者：
ELLEMAN, CJ;WILLSON, CE;DICKINSON, HG
通讯作者：
DICKINSON, HG
影响因子：
6.9
作者：
Chae K;Gonong BJ;Kim SC;Kieslich CA;Morikis D;Balasubramanian S;Lord EM
通讯作者：
Lord EM
影响因子：
4.6
作者：
Gremski, Kristina;Ditta, Gary;Yanofsky, Martin F.
通讯作者：
Yanofsky, Martin F.
影响因子：
64.5
作者：
CHEUNG, AY;WANG, H;WU, HM
通讯作者：
WU, HM