Within-Subject Reliability and between-Subject Variability of Oxidative Stress Markers in Saliva of Healthy Subjects: A Longitudinal Pilot Study.
Within-Subject Reliability and between-Subject Variability of Oxidative Stress Markers in Saliva of Healthy Subjects: A Longitudinal Pilot Study.
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DOI：
10.1155/2017/2697464
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Alajbeg I
中科院分区：
文献类型：
--
作者：
Alajbeg IZ;Lapić I;Rogić D;Vuletić L;Andabak Rogulj A;Illeš D;Knezović Zlatarić D;Badel T;Vrbanović E;Alajbeg I
本研究评估了健康成年人唾液氧化应激(OS)标志物的日变化和日波动。连续3天，分2次采集完整的非刺激性唾液。用分光光度法检测谷胱甘肽过氧化物酶(GPX)、超氧化物歧化酶(SOD)、总抗氧化能力(TAC)和尿酸(UA)，用免疫法测定8-羟基脱氧鸟苷(8-OHdG)和丙二醛(MDA)。唾液OS标志物在男性和女性之间没有显著差异。对于所有被检查的OS标志物，没有显示出显著的逐日变化。唾液中GPX、TAC和丙二醛水平有显著的日变化。对于SOD、TAC、GPX和UA，超过75%的受试者观察到良好到中等的个体内变异系数(CVs)。对于丙二醛和8-羟色胺，分别有60%和40%的受试者观察到个体内CVS >； 为35%。所有检查的OS标志物的受试者之间的差异都很大(CV%30.08%-85.70%)。由于唾液中丙二醛和8-羟色胺浓度的个体内变异性很大，这些标志物不能基于单次测量而可靠地验证，而几天内的多次测量将提供更可靠的信息。唾液中的SOD、TAC、GPX和UA在三天的测量中被证明是稳定的。试行注册。临床试验.gov NCT03029494。注册日期：2017-01-19。
The present study evaluated diurnal variations and day-to-day fluctuations of salivary oxidative stress (OS) markers in healthy adult individuals. Whole unstimulated saliva was collected at 2 time intervals over 3 consecutive days. Glutathione peroxidase (GPX), superoxide dismutase (SOD), total antioxidant capacity (TAC), and uric acid (UA) were analyzed using spectrophotometric methods, while 8-hydroxydeoxyguanosine (8-OHdG) and malondialdehyde (MDA) were determined using immunoassays. No significant differences for salivary OS markers between men and women were observed. For all examined OS markers, no significant day-to-day variations were demonstrated. Significant diurnal variations were found in salivary GPX, TAC and MDA levels. For SOD, TAC, GPX, and UA, good-to-moderate intraindividual coefficients of variations (CVs) were observed in more than 75% of the subjects. For MDA and 8-OHdG, intraindividual CVs > 35% were observed in 60% and 40% of the subjects, respectively. Between-subject variance was wide for all examined OS markers (CV% 30.08%–85.70%). Due to high intraindividual variability in the salivary concentrations of MDA and 8-OHdG, those markers cannot be reliably verified based on single measurements and multiple measurements over several days would provide more reliable information. Salivary SOD, TAC, GPX, and UA proved stable across three days of measurement. Trial Registration. ClinicalTrials.gov NCT03029494. Registered on 2017-01-19.
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