In vitro remineralization effects of grape seed extract on artificial root caries.
In vitro remineralization effects of grape seed extract on artificial root caries.
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DOI：
10.1016/j.jdent.2008.07.011
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发表时间：
2008-11
影响因子：
4.4
通讯作者：
Wu, Christine D.
中科院分区：
文献类型：
--
作者：
Xie, Qian;Bedran-Russo, Ana Karina;Wu, Christine D.
葡萄籽提取物（GSE）含有原花青素（PA），据报道，它可以通过增加胶原蛋白交联来加强胶原蛋白组织。我们使用体外pH循环模型来评价GSE对人工根龋再矿化的影响。将从牙根的颈部获得的完好的人牙齿碎片在37°C下储存在脱矿质溶液中96小时以诱导人工牙根龋损伤。然后将碎片分为三个处理组，包括：6.5% GSE、1，000 ppm氟化物（NaF）和对照组（未处理）。脱矿物质样品通过处理溶液、酸性缓冲液和中性缓冲液进行pH循环，每天6个循环，持续8天。随后使用显微硬度测试仪、偏振光显微镜（PLM）和共聚焦激光扫描显微镜（CLSM）对样品进行评估。使用ANOVA和Fisher检验分析数据（p<0.05）。与对照组相比，GSE和氟化物显著增加了病变的显微硬度（p<0.05）。PLM数据显示，与其他组相比，GSE处理的病变表面层上的矿物质沉淀带显著更厚（p>0.05），这由CLSM证实。我们的结论是，葡萄籽提取物积极影响人工根龋病变的脱矿和/或再矿化过程，最有可能通过不同的机制比氟化物。葡萄籽提取物可能是一种很有前途的非侵入性根龋治疗的天然制剂。
Grape seed extract (GSE) contains Proanthocyanidin (PA), which has been reported to strengthen collagen-based tissues by increasing collagen cross-links. We used an in vitro pH-cycling model to evaluate the effect of GSE on the remineralization of artificial root caries. Sound human teeth fragments obtained from the cervical portion of the root were stored in a demineralization solution for 96 hr at 37°C to induce artificial root caries lesions. The fragments were then divided into three treatment groups including: 6.5% GSE, 1,000 ppm fluoride (NaF), and a control (no treatment). The demineralized samples were pH-cycled through treatment solutions, acidic buffer and neutral buffer for 8 days at 6 cycles per day. The samples were subsequently evaluated using a microhardness tester; polarized light microscopy (PLM) and confocal laser scanning microscopy (CLSM). Data were analyzed using ANOVA and Fisher’s tests (p<0.05). GSE and fluoride significantly increased the microhardness of the lesions (p<0.05) when compared to a control group. PLM data revealed a significantly thicker mineral precipitation band on the surface layer of the GSE treated lesions when compared to the other groups (p>0.05), which was confirmed by CLSM. We concluded that grape seed extract positively affects the demineralization and/or remineralization processes of artificial root caries lesions, most likely through a different mechanism than that of Fluoride. Grape seed extract may be a promising natural agent for non-invasive root caries therapy.
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影响因子：
3
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