Diabetes Enhances IL-17 Expression and Alters the Oral Microbiome to Increase Its Pathogenicity.
Diabetes Enhances IL-17 Expression and Alters the Oral Microbiome to Increase Its Pathogenicity.
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DOI：
10.1016/j.chom.2017.06.014
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发表时间：
2017-07-12
影响因子：
30.3
通讯作者：
Graves DT
中科院分区：
文献类型：
--
作者：
Xiao E;Mattos M;Vieira GHA;Chen S;Corrêa JD;Wu Y;Albiero ML;Bittinger K;Graves DT
糖尿病是牙周炎的危险因素，牙周炎是一种炎症性骨骼疾病，也是成年人牙齿脱落的最大原因。糖尿病对肠道微生物群有显著影响，但口腔研究尚未得出结论。通过16 s rRNA测序，我们发现糖尿病导致口腔细菌组成的变化，并且通过转移到无菌小鼠，糖尿病小鼠的口腔微生物群更具致病性。此外，用IL-17抗体治疗降低了糖尿病小鼠中口腔微生物群的致病性;当转移到受体无菌小鼠时，来自IL-17治疗的供体的口腔微生物群诱导减少的中性粒细胞募集、减少的IL-6和RANKL以及更少的骨吸收。因此，糖尿病增强的IL-17改变了口腔微生物群，使其更具致病性。我们的研究结果为更好地了解糖尿病如何增加牙齿脱落的风险和严重程度提供了机制基础。糖尿病会增加牙周病，这是牙齿脱落的主要原因。Xiao等人通过转移到无菌小鼠中证明，来自糖尿病小鼠的口腔微生物群比来自正常小鼠的微生物群诱导更多的牙周炎症和骨丢失。糖尿病通过IL-17介导的机制增加了口腔微生物群的致病性。
Diabetes is a risk factor for periodontitis, an inflammatory bone disorder and the greatest cause of tooth loss in adults. Diabetes has a significant impact on the gut microbiota, however studies in the oral cavity have been inconclusive. By 16s rRNA sequencing we show here that diabetes causes a shift in oral bacterial composition and, by transfer to germ free mice, that the oral microbiota of diabetic mice is more pathogenic. Furthermore, treatment with IL-17 antibody decreases the pathogenicity of the oral microbiota in diabetic mice; when transferred to recipient germ-free mice oral microbiota from IL-17-treated donors induced reduced neutrophil recruitment, reduced IL-6 and RANKL, and less bone resorption. Thus, diabetes-enhanced IL-17 alters the oral microbiota and renders it more pathogenic. Our findings provide a mechanistic basis to better understand how diabetes can increase the risk and severity of tooth loss. Diabetes increases periodontal disease, a major cause of tooth loss. Xiao et al. demonstrated, by transfer to germ-free mice, that oral microbiota from diabetic mice induced more periodontal inflammation and bone loss than microbiota from normal mice. Diabetes increased the pathogenicity of the oral microbiota through an IL-17 mediated mechanism.
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DOI：
10.1073/pnas.1004204107
发表时间：
2010-08-17
影响因子：
11.1
作者：
Grice, Elizabeth A.;Snitkin, Evan S.;Segre, Julia A.
通讯作者：
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影响因子：
7.6
作者：
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作者：
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影响因子：
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作者：
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通讯作者：
Tomas, I.
影响因子：
11
作者：
Griffen, Ann L.;Beall, Clifford J.;Leys, Eugene J.
通讯作者：
Leys, Eugene J.