Acquisition of an Inverted Microscope with Video Camera and Time Lapse Recorder

购买带有摄像机和延时录像机的倒置显微镜

• 批准号: 8613929
• 负责人: William Wilfinger
• 金额: --
• 依托单位: University of Cincinnati Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-11-01 至 1990-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8613929&HistoricalAwards=false
• 关键词: Acquisition; Inverted; Microscope; Video; Camera

This proposal requests funds for the purchase of a Zeiss inverted microscope and accessories. The microscope will be used to: l. Evaluate the cytostructural and secretory properties of cultured rat pituitary cells in order to determine whether subpopulations of gonadotropin secreting cells are differentially recruited to release hormone during periods of continuous hormonal stimulation, 2. Quantitate changes in endothelial cell morphology and migration behavior in response to activated human peripheral blood mononuclear cell factor, 3. Identify and study ion channel distributions on cultured myocardial and vascular smooth muscle cells, 4. Study the transfer of fluorescent dyes between coupled myocardial cells and to obtain cellular morphometric data, and 5. Evaluate the functional properties of acetylcholine containing ventral horn neurons. The microscope and accessories will benefit the faculty of the Department of Physiology and will allow the department to expand and broaden its basic research potential in electrophysiology.

该提案要求资金用于购买蔡司倒置显微镜和配件。 显微镜将用于： l． 评估培养的大鼠垂体细胞的细胞结构和分泌特性，以确定在连续激素刺激期间，促性腺激素分泌细胞亚群是否被差异性地募集以释放激素，2. 定量响应激活的人外周血单核细胞因子的内皮细胞形态和迁移行为的变化，3. 识别和研究培养心肌上的离子通道分布 和血管平滑肌细胞，4.研究偶联心肌细胞之间荧光染料的转移并获得细胞形态测量数据，以及5.评估含有乙酰胆碱的腹角神经元的功能特性。 显微镜和配件将使生理学系的教师受益，并使该系能够扩大和拓宽其在电生理学方面的基础研究潜力。