High throughput gentle imaging of cells, embryos and organoids with an inverted lattice light sheet microscope.

使用倒置点阵光片显微镜对细胞、胚胎和类器官进行高通量温和成像。

• 批准号: BB/V018973/1
• 负责人: Kevin O'Holleran
• 金额: $ 89.01万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FV018973%2F1
• 关键词: throughput; gentle; imaging; cells; embryos

The Cambridge Advanced Imaging Centre (CAIC) is a bioimaging research facility at the University of Cambridge. It provides researchers across the region, nationally and internationally with access to state-of-the-art microscopes. CAIC enhances the value generated by microscopes by acting as a hub for physicists, engineers, computer scientists, chemists and biologists to work together. Since opening in 2013, CAIC has kept pace with technology advances by directly developing microscopes and improving access to equipment among departments, institutes and industry. In order to maintain excellence, CAIC continually identifies and implements cutting-edge imaging methods that meet the pressing needs of the region's imaging community.In this project we will install a novel inverted lattice light sheet microscope into CAIC. This equipment will fill a critical gap in our national live imaging portfolio, establishing a capability that will deliver world leading excellence in live imaging of cells and embryos. The hallmark of light sheet microscopy is a significant reduction in damage caused by light to the specimen under observation when compared to gold-standard confocal microscopes. This benefit is offset by reduced resolution and cumbersome sample mounting protocols which limit use. At CAIC we are keenly aware of these limitations, having developed two customised upright and horizontal light sheet microscopes. These barriers to widespread adoption have been eliminated by a novel microscope - the inverted lattice light sheet microscope. This new technology has an innovative lens system, making light sheet microscopes compatible with all common microscope slides and dishes, the impact of which is enormous.It is difficult to over-emphasise the importance of low-light illumination in fluorescence microscopy - particularly for research projects that require long-term imaging of organoids and embryos. Researchers will finally be able to exploit the gentle imaging of light sheet microscopy in an unrestricted way, using multi-well plates, grids and slides to image an order of magnitude more specimens than before. Enabling low phototoxicity imaging of organoids and embryos will drastically improve success rates in imaging, reducing the total number of animals required, making this microscope an essential tool in achieving the 3Rs in research using animals. The ability to easily image many samples in long-term imaging experiments removes selection bias and increases sample numbers by a factor of ten, leading to greatly improved data driven biology. The inverted lattice light sheet microscope will complement our existing live imaging equipment portfolio, resulting in a unique live bioimaging facility with world-leading capabilities.

剑桥高级成像中心（CAIC）是剑桥大学的生物成像研究机构。它为整个地区的研究人员提供了国家和国际上最先进的显微镜。CAIC通过充当物理学家、工程师、计算机科学家、化学家和生物学家共同工作的中心，提高了显微镜产生的价值。自2013年开业以来，CAIC通过直接开发显微镜和改善部门，研究所和行业之间的设备访问，跟上了技术进步的步伐。为了保持卓越，CAIC不断识别和实施尖端的成像方法，以满足该地区成像社区的迫切需求。在这个项目中，我们将安装一个新颖的倒置点阵光片显微镜到CAIC。该设备将填补我们国家活体成像产品组合的关键空白，建立一种在细胞和胚胎活体成像方面提供世界领先的卓越能力。光片显微镜的特点是与金标准共聚焦显微镜相比，观察下的光对标本造成的损伤显着减少。这一优势被分辨率降低和限制使用的繁琐样品安装协议所抵消。在CAIC，我们敏锐地意识到这些限制，开发了两种定制的直立和水平光片显微镜。这些广泛采用的障碍已经被一种新的显微镜-倒置晶格光片显微镜所消除。这项新技术具有创新的透镜系统，使光片显微镜与所有常见的显微镜载玻片和培养皿兼容，其影响是巨大的。在荧光显微镜中，很难过分强调弱光照明的重要性-特别是对于需要对类器官和胚胎进行长期成像的研究项目。研究人员最终将能够以不受限制的方式利用光片显微镜的温和成像，使用多孔板，网格和载玻片来成像比以前多一个数量级的标本。实现类器官和胚胎的低光毒性成像将大大提高成像的成功率，减少所需的动物总数，使这种显微镜成为在使用动物的研究中实现3R的重要工具。在长期成像实验中轻松成像许多样本的能力消除了选择偏差，并将样本数量增加了十倍，从而大大改善了数据驱动的生物学。倒置点阵光片显微镜将补充我们现有的活体成像设备组合，从而形成具有世界领先能力的独特活体生物成像设施。