Inverted confocal microscope for life science

用于生命科学的倒置共焦显微镜

• 批准号: 517006066
• 金额: --
• 依托单位: Georg-August-Universität Göttingen
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/517006066?language=en
• 关键词: Inverted; confocal; microscope; life; science

The replacement of an inverted confocal microscope is requested. It is planned to procure a versatile instrument that will serve the many diverse third-party funded projects in the field of cell- and membrane biophysics, teaching as well as general cell culture tasks. These include immunostaining of cells and biomimetic systems (cytoskeleton, membrane proteins), live cell imaging (wound healing, epithelial-to-mesenchymal transition), measurements of diffusion, 3D imaging of organoids, high-resolution imaging of cytoskeletal networks (artificial and within cells), combination with an existing atomic force microscope, a nanomanipulator or microinjection setup. The participating research groups work on biomimetic systems such as vesicles, microcompartments, microgels, and solid-supported lipid membranes, as well as biological systems such as cell membranes, adherent cells, and organoids. Confocal fluorescence microscopy studies are minimal invasive to the samples being studied and the technique is relatively easy to extend to new experiments and samples covering a large parameter space. The demand for fluorescence microscopy in biophysical research studies has increased tremendously in recent years, so that there are hardly any projects in which fluorescence microscopy does not play a central role. The existing inverted confocal microscope of the Faculty of Chemistry has reached the end of its service life and must therefore be urgently replaced by a new device in order to ensure the continuation of a large number of research projects in the future. Furthermore, new developments in measurement and detector technology allow the design of new experiments, which cannot be performed with the existing equipment. Novel GaAsP detectors improve the signal-to-noise ratio by a factor of 2, which increases the possibilities for long-term measurements, the sensitivity and the quantifiability of the data. Techniques are available that offer increasing spatial resolution, while simultaneously improving sensitivity. This is especially important for delicate samples such as cytoskeletal filaments and living cells. In addition, methods like Fluorescence Correlation Spectroscopy (FCS) and lifetime measurements (FLIM) are invaluable tools for the investigation of lipid and protein diffusion as well as to explore the mechanical properties of cell membranes with modern fluorophores that report on the tension of cells.

要求更换倒置共焦显微镜。计划采购一种多功能仪器，用于细胞和膜生物物理学领域的许多不同的第三方资助项目，教学以及一般细胞培养任务。这些包括细胞和仿生系统（细胞骨架，膜蛋白）的免疫染色，活细胞成像（伤口愈合，上皮-间充质转化），扩散测量，类器官的3D成像，细胞骨架网络（人工和细胞内）的高分辨率成像，与现有的原子力显微镜，纳米操纵器或显微注射装置的组合。参与的研究小组致力于仿生系统，如囊泡，微室，微凝胶和固体支持的脂质膜，以及生物系统，如细胞膜，粘附细胞和类器官。共焦荧光显微镜研究是最小的侵入性的样品正在研究和技术是相对容易扩展到新的实验和样品覆盖一个大的参数空间。近年来，生物物理研究中对荧光显微镜的需求大幅增加，因此几乎没有荧光显微镜不发挥核心作用的项目。化学学院现有的倒置共聚焦显微镜已达到使用寿命，因此必须紧急更换新设备，以确保未来大量研究项目的继续。此外，测量和检测器技术的新发展允许设计新的实验，而这些实验无法使用现有设备进行。新型GaAsP探测器将信噪比提高了2倍，这增加了长期测量的可能性，数据的灵敏度和可量化性。技术是可用的，提供增加的空间分辨率，同时提高灵敏度。这对于细胞骨架丝和活细胞等精细样品尤其重要。此外，荧光相关光谱（FCS）和寿命测量（FLIM）等方法是研究脂质和蛋白质扩散以及利用现代荧光团探索细胞膜机械特性的宝贵工具，可报告细胞张力。