High Performance Liquid Chromatography in an Integrated Advanced Laboratory Curriculum

集成高级实验室课程中的高效液相色谱

• 批准号: 8651051
• 负责人: Stephen Walsh
• 金额: $ 1.77万
• 依托单位: Manhattan College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-15 至 1988-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8651051&HistoricalAwards=false
• 关键词: Performance; Liquid; Chromatography; Integrated; Advanced

A high performance liquid chromatography system will be used to teach principles of HPLC to undergraduate science and engineering students at Manhattan College and the College of Mount St. Vincent. The system will be used in four integrated, upper-division laboratory courses. In these courses, students develop skills and techniques in the analytical, instrumental, physical, synthetic, and biochemical areas. Experiments are sequenced so that basic skills are learned first, with a progression toward sophisticated, project-oriented work in the final course. The project involves both the use of previously tested protocols and the development of new experiments. Their object will be teaching students the principles and applications of HPLC. Several specific chromatographic modes will be used: normal phase, reverse phase, ion exchange, and gel permeation. In addition, several types of detectors will be used: UV/visible variable wavelength absorbance, refractive index, fluorescence, and electrochemical. The curriculum is designed so that fundamental principles are learned in the early labs, while students in the later courses use the HPLC as an investigative tool for topics such as kinetics.

高效液相色谱系统将用于向曼哈顿学院和圣文森特山学院的理工科本科生教授 HPLC 原理。 该系统将用于四个综合的高级实验室课程。 在这些课程中，学生培养分析、仪器、物理、合成和生物化学领域的技能和技术。 实验按顺序进行，以便首先学习基本技能，然后在最终课程中逐步进行复杂的、以项目为导向的工作。 该项目涉及使用先前测试的协议和开发新实验。 他们的目标是教授学生 HPLC 的原理和应用。 将使用几种特定的色谱模式：正相、反相、离子交换和凝胶渗透。 此外，还将使用多种类型的检测器：紫外/可见光可变波长吸光度、折射率、荧光和电化学。 该课程的设计是为了让学生在早期的实验中学习基本原理，而在后期课程中，学生则使用 HPLC 作为动力学等主题的研究工具。