Differentiation of the Interstitial Cell Line in Hydrozoan Planulae

浮肿水螅间质细胞系的分化

• 批准号: 8702212
• 负责人: Vicki Martin
• 金额: $ 17.2万
• 依托单位: University of Notre Dame
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1990-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8702212&HistoricalAwards=false
• 关键词: Differentiation; Interstitial; Cell; Line; Hydrozoan

Dr. Martin will examine the development and differentiation of undifferentiated cells, interstitial cells, during embryogenesis in a marine hydrozoan, Halocordyl disticha. Hydrozoan planulae contain a population of interstitial cells which either divide to maintain the I- cell population or differentiate into ganglionic nerve cells or nematocytes. Embryos treated with colchicine or hydroxyurea have either no or reduced numbers of I cells respectively. Five major aspects of I- cell behaviour in larval tissues will be analyzed: 1) the dynamics of larval stem cell repopulation in epithelial planulae; 2) the involvement of I-cells and their derivatives in metamorphosis; 3) a determination if larval I-cells exhibit restrictions in their developmental potential with time; 4) an examination of the stem cell properties and developmental potential of a larval population of hydroxyurea resistant interstitial cells; and 5) a comparison of the larval I-cell population to the adult I-cell population. Pieces of different treated or aged control embryos will be grafted to epithelial planulae and removed at various times after grafting for analysis. The general problem approached by this research is how the spatial pattern of differentiated cells is established during embryogenesis and how such an organization is carried over and maintained in the adult state. Dr. Martin is studying cellular commitment, proliferation and differentiation of a manipulatable population of stem cells in a marine hydrozoan as a model.

马丁博士将研究 未分化的细胞，间质细胞，在胚胎发生过程中， 海洋水螅，Halocordyl disticha。 扁平水螅虫含有 间质细胞群，其分裂以维持I- 细胞群或分化成神经节神经细胞，或 巨噬细胞 用秋水仙素或羟基脲处理的胚胎 I细胞分别为无或减少。 五个主要方面的I- 将分析幼虫组织中的细胞行为：1） 幼虫干细胞在上皮浮浪中的再增殖; 2）参与 I-细胞及其衍生物在变态; 3）确定， 幼虫I-细胞在其发育潜能方面表现出限制 随着时间的推移; 4）检查干细胞特性， 抗羟基脲幼虫群体发育潜力 间质细胞;和5）幼虫I-细胞群的比较 成人I细胞群。不同处理或老化的工件 将对照胚胎移植到上皮浮凸上，并在 用于分析。 本研究探讨的一般问题是， 分化细胞的模式在胚胎发生期间建立， 这种组织是如何在成年人身上延续和维持的 状态 马丁博士正在研究细胞的承诺，增殖和 干细胞的可操作群体在海洋中的分化 水螅虫作为模型。